FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_n01_10x_ctr2_dec18_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_n01_10x_ctr2_dec18_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66107215
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCCCATCACGATGAAATTTCAAAGA667700.1010025910182421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC448750.020.0005231
ATAACGC429400.019.5688421
TGGGCGT363850.019.1831911
GGGCGTG259950.019.1823842
ACTGCTC456200.018.9142041
CACGAGA296450.018.8791928
AGCTGAA320700.018.8628188
ACGAGAG296400.018.8576079
TGCGACG226800.018.7971910
GTTGCAC324300.018.7037164
CATATTC474900.018.6957531
TGAGCCG176700.018.6865391
GAACCAC256050.018.6536454
GCACGTC329850.018.6482857
TGAACCA299900.018.6396753
ATGGCGA151250.018.6327672
CAATGGA230100.018.6096538
GCAGCGA225250.018.4932376
TGACGGC171300.018.4479311
TCTGCGA232100.018.4221488
Produced by FastQC (version 0.11.5)