FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_n01_10x_ctr2_dec18_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_n01_10x_ctr2_dec18_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37989980
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC263000.019.8742281
ATAACGC240150.019.5174311
TGGGCGT203800.019.0924841
GGGCGTG146600.019.051712
TGAGCCG99900.018.9438251
GCACGTC183550.018.9358627
ACGAGAG167950.018.9191959
CACGAGA168050.018.9079158
TGAACCA173700.018.7928893
TGCGACG131350.018.7787510
ACTGCTC259750.018.7360861
GTTGCAC183000.018.727054
CATATTC275550.018.713691
GCAGCGA124200.018.7104366
ATGGCGA90400.018.6652492
CGGCCGA85650.018.6472859
TTGCACG176200.018.621495
AGCTGAA179450.018.6078788
TGACGGC96050.018.5332131
GAACCAC150150.018.5096824
Produced by FastQC (version 0.11.5)