FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_n01_10x_ctr2_dec18_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_n01_10x_ctr2_dec18_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41239912
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC282450.020.0152471
ATAACGC266900.019.446091
TGGGCGT228450.019.1792341
CACGAGA185000.019.0997078
GGGCGTG165500.019.0712912
TGCGACG146350.018.98508610
ACGAGAG186350.018.9669769
ACTGCTC286750.018.920351
CATATTC295950.018.8147961
GTTGCAC201350.018.7831334
AGCTGAA193200.018.7510388
GCAGCGA139350.018.718396
GCACGTC205850.018.715727
ATGGCGA95450.018.7008822
TCTGCGA149300.018.6450398
TGAACCA188450.018.6315163
AAAGGGC152150.018.620616
GAACCAC161800.018.5853374
CAATGGA136500.018.5779518
TGAGCCG111350.018.4860441
Produced by FastQC (version 0.11.5)