FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455167733
Sequences flagged as poor quality0
Sequence length99
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32425840.7123932047265749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT26776950.078.3656541
AGCAGTG27551350.075.970342
GCAGTGG33326650.062.7499963
AGTGGTA34874650.060.0203445
CAGTGGT34929250.059.876954
GTGGTAT37845850.055.3045166
GGTATCA39097050.053.4869848
TGGTATC39674600.052.7243167
GTATCAA40398350.051.7806329
ACGCAGA37708900.030.950714-15
CAACGCA37836000.030.82171612-13
ATCAACG37988900.030.7384910-11
GCAGAGT38376050.030.38499516-17
AGTACAT38376800.030.22181920-21
AGAGTAC39318150.029.63220818-19
TACATGG39121000.029.54152722-23
CATGGGG21805250.029.29549824-25
CGCAGAG38030250.027.58010716-17
TCAACGC38130550.027.45044312-13
AACGCAG38433850.027.29997414-15
Produced by FastQC (version 0.11.5)