FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455167733
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCC2793300.019.4301281
CGAGCCG1400350.018.673562
GCATGCG2190500.018.657011
ATAACGC1943800.018.61591
TGACGGC1541700.018.4383661
TGGGCGT1556450.018.41211
ATGCGAG953950.018.3885483
ACTGAAC1724500.018.3793641
GCGCGAT1352350.018.372231
CGGACTG1623500.018.3575481
TGAGAGG2201300.018.3464571
AGCAACA2272700.018.3054263
GTAACTC2164900.018.2789553
CGAGAAG2183550.018.064581
CGATTGA1936450.018.0524641
GAGGAGA935450.018.0324754
CTCCGTA2436250.018.0301827
TCCGTAG2409100.017.9353378
GTAAACG2028650.017.9344758
AGAGCGA1358100.017.8806651
Produced by FastQC (version 0.11.5)