FastQCFastQC Report
Tue 9 Oct 2018
HG5YYBGX7_n02_ba148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5YYBGX7_n02_ba148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495804074
Sequences flagged as poor quality0
Sequence length99
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79456021.6025689212065652No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG8700340.17547939712976218No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAACAAGCGAGATAGGAA8419440.16981385271957244No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCA8271250.16682497046202166No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG5787100.11672150963406565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT26262400.078.43431
GTGGTAT27389550.074.9236
GGTATCA27403250.074.845518
GTATCAA27966500.073.331639
AGCAGTG28445550.072.215842
TGGTATC28571250.071.743347
AGTGGTA28841150.071.2389765
GCAGTGG31172050.065.929933
CAGTGGT31971300.064.313744
ATCAACG26611950.041.80451610-11
CAACGCA27349850.040.70864512-13
ACGCAGA27732450.040.18794314-15
AGTACAT27819750.039.72838220-21
AGAGTAC28537600.038.91227718-19
TACATGG28171600.038.87413422-23
TCAACGC27021000.037.99871412-13
GCAGAGT29412500.037.84675216-17
CGCAGAG27278750.037.7875316-17
TATCAAC27272100.037.74336210-11
AACGCAG27910450.036.88075314-15
Produced by FastQC (version 0.11.5)