FastQCFastQC Report
Mon 8 Oct 2018
HG5YYBGX7_n01_ba148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5YYBGX7_n01_ba148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495804074
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGGA1356300.018.6184041
GGCTCGA1786750.018.5637821
GCGCGAT1346600.018.3765451
TGCGAGA809600.018.1939074
CCGGGAT1600100.018.171061
CAATGGA1556350.018.1298798
ACGAGCC1513750.018.0703181
GGAAAGC1384000.018.0104921
GCCGGAG776000.017.9601613
CCACTAC1879050.017.895081
TGGGCGT1328000.017.892021
GGGAATG1200300.017.8362121
GGATTAC1690150.017.7759151
ACGGAGA1603650.017.6232171
CTCGAAA1329950.017.5585581
CGGACTG1282350.017.5255491
CTAAGAC1715400.017.490381
CCAGCGA1413000.017.463931
GGGCATC1222750.017.4168191
GACTAAC1313050.017.3949741
Produced by FastQC (version 0.11.5)