FastQCFastQC Report
Thu 6 Oct 2016
HG5MKAFXX_n01_gln3_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5MKAFXX_n01_gln3_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7993658
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCGAAGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC5257986.5776894633220495No Hit
TATCGAAGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT2194132.7448384706976454No Hit
TATCGATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC1358601.6995973558038133No Hit
TATCGAAGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG1254011.568756131423186No Hit
TATCGAAGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA1244371.5566965712068241No Hit
TATCGAAGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT1124991.4073531792328369No Hit
TATCGAAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT839941.0507579883953004No Hit
TATCGAACCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG548280.6858937422641799No Hit
TATCGAAGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTC517430.6473006475883757No Hit
TATCGATGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT515550.6449487831478404No Hit
TATCGAACGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT508040.6355538353029364No Hit
TATCGAAGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC471750.5901553456502643No Hit
TATCGAAGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG463810.5802224713641739No Hit
TATCGAACGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT410160.5131067653882615No Hit
TATCGATCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT334560.41853179107737665No Hit
TATCGATGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG280350.35071552973619835No Hit
TATCGATGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA265710.33240101090139207No Hit
TATCGAAGGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCG259330.32441968370425656No Hit
TATCGAAGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTC213410.26697414375245976No Hit
TATCGAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCG210220.262983480153892No Hit
TATCGAAGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGA194710.2435805985194763No Hit
TATCGATCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT187070.2340230217504927No Hit
TATCGAAATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC185600.23218406391666996No Hit
TATCGATATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC163000.20391165096129957No Hit
TATCGATGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTC133870.16747026205024032No Hit
TATCGATGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG127770.1598392125357377No Hit
TATCGAAGGGGGTATGAACAACAATTGTTGTAGTCGCAACTACGGTAATT125370.1568368323988842No Hit
TATCGAACTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT124700.1559986679440126No Hit
TATCGAAGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGAT122530.1532840159036076No Hit
TATCGAATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCA117480.14696650769897837No Hit
TATCGAAATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC113320.1417623821284323No Hit
TATCGATGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT112200.1403612713979007No Hit
TATCGATGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT107290.13421890203458792No Hit
TATCGATTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA102480.12820163184364403No Hit
TATCGAACCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGC101870.1274385268921938No Hit
TATCGAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACT94490.11820620797136931No Hit
TATCGATGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC93780.11731800384755015No Hit
TATCGAAGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGT93540.1170177658338648No Hit
TATCGATCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT93140.11651736914438922No Hit
TATCGAAGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGAC82690.10344450563183966No Hit
TATCGATATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC81920.10248124200459914No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAA6001150.0144.500231
CGAAGGG585100.0144.169954
TCGAAGC1021850.0144.053073
TCGAACG583450.0143.944063
TCGAAGT1115600.0143.75373
CGAAGTG712150.0143.743624
AGTGCGT560450.0143.719777
ATCGAAG3557450.0143.609792
GAAGTGC592650.0143.58095
GAAGGGC364650.0143.325325
CGAACGC268850.0143.007024
AAGTGCG574050.0143.005396
AGGGCGC234200.0142.591117
GAACGCG96450.0141.969015
GAAGGCG258350.0141.580255
GAAGGGG153650.0141.484155
CGAACGG131650.0141.341234
TCGAAGG1273500.0141.076233
CGATCGC172200.0140.976234
CGAAGCC309700.0140.832434
Produced by FastQC (version 0.11.2)