FastQCFastQC Report
Thu 6 Oct 2016
HG5MKAFXX_n01_gln3_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5MKAFXX_n01_gln3_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9408823
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTAAGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC5496205.841538309308189No Hit
AGTCTAAGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT2030882.15848464786722No Hit
AGTCTAAGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA1648051.7516005987146321No Hit
AGTCTAACGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT1398851.4867428157592082No Hit
AGTCTAAGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT1343111.4275005492185366No Hit
AGTCTATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC1218631.2951991976042063No Hit
AGTCTAAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT987771.0498337571022431No Hit
AGTCTATCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT934730.9934611374876539No Hit
AGTCTAATGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA669280.7113323313659955No Hit
AGTCTAAGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGC623630.6628140416713122No Hit
AGTCTAAGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGT593400.6306846244211417No Hit
AGTCTAAGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA504610.5363157538408364No Hit
AGTCTAAGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG438270.46580746603480583No Hit
AGTCTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG424140.45078964712164316No Hit
AGTCTAATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCA420780.4472185309469633No Hit
AGTCTATGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT406050.4315630127168935No Hit
AGTCTAAGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTC403020.4283426311665125No Hit
AGTCTAACGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT331510.35233950091313226No Hit
AGTCTAAATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC306910.32619382891994037No Hit
AGTCTATGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG300520.31940233119487954No Hit
AGTCTATGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA295760.31434324994741636No Hit
AGTCTAATCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCAT283470.30128104227276886No Hit
AGTCTAAATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC269920.28687966603261644No Hit
AGTCTAAACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCT256950.27309473246547417No Hit
AGTCTATATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC211670.2249696906828835No Hit
AGTCTAAGTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAA203020.21577619219747252No Hit
AGTCTAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCG198470.21094030571092684No Hit
AGTCTATATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC195120.20737981785819543No Hit
AGTCTAACTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGATAGACT178150.1893435555116724No Hit
AGTCTAAGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAA171340.18210566826477656No Hit
AGTCTAACTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT166470.17692967547588045No Hit
AGTCTAAGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTT160250.1703188592239433No Hit
AGTCTAAGGAACTATCAAATAAACGATAACTGATTTAATGAGCCATTCGC154270.16396312269876903No Hit
AGTCTAACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT146770.15599188123743002No Hit
AGTCTATCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT143730.1527608713651006No Hit
AGTCTAATTTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC132790.1411334871534941No Hit
AGTCTAAGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGA132150.1404532745487932No Hit
AGTCTAAACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGCGT126450.13439513103817555No Hit
AGTCTAACGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA124640.13247140476550576No Hit
AGTCTATGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA122760.13047328023919677No Hit
AGTCTAAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCG121740.12938919140045466No Hit
AGTCTATCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGC120010.12755049170337246No Hit
AGTCTATCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT119720.12724227036686736No Hit
AGTCTAAGTTCTGCTTACCAAAAATGGCCCACTAAAAGCTCTTCATTCAA116670.1240006321725895No Hit
AGTCTAAGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTC115750.12302282655333191No Hit
AGTCTAAGGGAGTTGCTCTAGCTCTTCTTTTCCCCTCTGATCACCTACGG114090.12125852510988888No Hit
AGTCTATTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA112420.1194835953444974No Hit
AGTCTAAGGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA111830.11885652434953872No Hit
AGTCTAATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCG107940.11472210711159089No Hit
AGTCTAAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAG104490.11105533603937495No Hit
AGTCTATGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT96490.10255267848061336No Hit
AGTCTATGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG95840.10186183755396397No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAA7147500.0144.870161
CTAAGCG617550.0144.639974
GTCTATG708600.0144.489352
AGTCTAT2236400.0144.40881
TAAGTGC601150.0144.377465
CTAACGC333250.0144.327824
CTAAGTG707700.0144.201924
GTCTAAC2052600.0143.9882
TCTAACC802550.0143.833273
AGTGCGT566850.0143.827157
TCTAACG769750.0143.671373
AAGTGCG580400.0143.581546
TAAGGCG316000.0143.558125
TAACCCC252200.0143.485755
AGGGCGC216550.0143.471737
TCTATCG427100.0143.317063
AAGGCGC197800.0142.891436
TCTAAGC1383350.0142.858113
TAACGCT128750.0142.615665
GTCTATC1202000.0142.531892
Produced by FastQC (version 0.11.2)