FastQCFastQC Report
Thu 6 Oct 2016
HG5MKAFXX_n01_gln3_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5MKAFXX_n01_gln3_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6178423
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAAGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC3333365.395163134670449No Hit
GACTTAAGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT1452592.35106919678371No Hit
GACTTAAGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA1169921.8935576278930726No Hit
GACTTAAGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG1117621.8089081955055522No Hit
GACTTAAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT1095661.7733651451187462No Hit
GACTTAAGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA947461.5334981110875703No Hit
GACTTAAGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT818801.3252572703422865No Hit
GACTTAACCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG614920.9952701522702476No Hit
GACTTAACGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC607420.9831311323293986No Hit
GACTTATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC576830.933620116330656No Hit
GACTTAAGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC538380.8713874074339035No Hit
GACTTAAGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG528620.8555904961508787No Hit
GACTTAACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCAC490700.7942156113299462No Hit
GACTTAAGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG461770.7473913650781113No Hit
GACTTAACGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT456800.7393472411973088No Hit
GACTTAAGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA411530.6660761168343443No Hit
GACTTAAGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGC371820.6018040525875292No Hit
GACTTAACCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG357920.5793064022971558No Hit
GACTTAAGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGAT357210.5781572417427554No Hit
GACTTAATGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA353270.5717802099338294No Hit
GACTTAAGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTC315060.5099359496751842No Hit
GACTTATCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCAC259080.41933030483668726No Hit
GACTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG242350.3922521976886335No Hit
GACTTAACGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC241880.3914914857723403No Hit
GACTTATGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT240650.38950068650204106No Hit
GACTTATCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC238380.3858266097999441No Hit
GACTTAAGCCGACTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGT234660.379805655909283No Hit
GACTTAAGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGAT234350.3793039097517279No Hit
GACTTAACGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT204770.3314276151050195No Hit
GACTTAACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGA202810.32825528456047764No Hit
GACTTATCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT192690.3118757003202921No Hit
GACTTAAGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTT186690.3021644843676129No Hit
GACTTAACCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGC185900.30088584093384346No Hit
GACTTAACCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACT185770.30067543125486873No Hit
GACTTAAGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTT181390.29358624360941293No Hit
GACTTAACCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTT177100.2866427242032473No Hit
GACTTAAGTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAA174540.28249927206343756No Hit
GACTTAAACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCT173250.28041136063361155No Hit
GACTTAATTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTAC170010.2751673040191648No Hit
GACTTAACTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT168930.2734192851476825No Hit
GACTTATGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA163600.26479248830971913No Hit
GACTTAAGAATTACCGCGGTTATACCATGTAGTAAAGGAACTATCAAATA156580.2534303656450845No Hit
GACTTATGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA153430.24833197726992795No Hit
GACTTAACACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCC152030.24606602688096946No Hit
GACTTAAGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTT150830.24412378369043364No Hit
GACTTATGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG149520.24200350154076536No Hit
GACTTATCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG141740.22941129152212464No Hit
GACTTAAGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC139920.22646555601647864No Hit
GACTTATGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT122590.19841632727315692No Hit
GACTTAATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCA120550.195114513849246No Hit
GACTTAACAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATT118080.19111672994872642No Hit
GACTTAACCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAA109670.1775048422550544No Hit
GACTTAAGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTT108480.1755787844244397No Hit
GACTTAACCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC104410.168991342936539No Hit
GACTTAAGCCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACA101170.16374728632209223No Hit
GACTTAAGCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCA100160.16211256497005788No Hit
GACTTAAGGGAGTTGCTCTAGCTCTTCTTTTCCCCTCTGATCACCTACGG99330.16076918009660393No Hit
GACTTAAATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC99120.16042928753826016No Hit
GACTTAAGGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCG99080.16036454609857564No Hit
GACTTAACCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCC98130.1588269369060681No Hit
GACTTATGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT98020.15864889794693565No Hit
GACTTATCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGA96990.15698180587505906No Hit
GACTTAATCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCAT95340.15431122148807228No Hit
GACTTATCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC94610.15312969021382966No Hit
GACTTAAGCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAAC93480.15130074454274173No Hit
GACTTAACGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTAT93020.15055621798636967No Hit
GACTTAAGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGA91450.14801511647875193No Hit
GACTTAAGCCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCT91060.1473838874418278No Hit
GACTTAAGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCT89960.14560349785050328No Hit
GACTTAACCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCAT88820.14375836681949425No Hit
GACTTAACTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGC84560.136863403493092No Hit
GACTTAAGTCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCA84370.13655588165459048No Hit
GACTTAACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT83570.13526105286089996No Hit
GACTTAACCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACG82770.13396622406720937No Hit
GACTTAACCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTTC81350.13166790295840863No Hit
GACTTAACTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGATAAGTC81150.13134419575998602No Hit
GACTTATACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCT81060.13119852752069583No Hit
GACTTATCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT80730.13066441064329845No Hit
GACTTATCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT80600.13045400096432375No Hit
GACTTAAGCGGTTATCAGTACGACCTGGCATGAAAACTATTCCTTCCTGT78900.1277024897777313No Hit
GACTTAACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTT76980.12459490067287396No Hit
GACTTAACCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTG76700.12414171059508226No Hit
GACTTAACCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGT76500.12381800339665965No Hit
GACTTAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCG76260.12342955475855247No Hit
GACTTAAGGAACTATCAAATAAACGATAACTGATTTAATGAGCCATTCGC75560.12229657956407322No Hit
GACTTATGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG74470.12053237533266985No Hit
GACTTAACCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGG73790.11943177085803286No Hit
GACTTATCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGC72730.11771612270639288No Hit
GACTTATCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATT72130.11674500111112496No Hit
GACTTAATGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC72030.11658314751191363No Hit
GACTTAACCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTA71330.1154501723174344No Hit
GACTTAACAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT70700.11443049464240308No Hit
GACTTATGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG68720.11122579337801897No Hit
GACTTAAGCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGG67650.10949395986645782No Hit
GACTTAAGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAG67530.10929973554740424No Hit
GACTTATATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC67020.10847428219142652No Hit
GACTTAAGCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAA66210.10716326803781483No Hit
GACTTAAGCTGCGGTTATCAGTACGACCTGGCATGAAAACTATTCCTTCC65500.10601410748341447No Hit
GACTTAACGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCT65380.10581988316436088No Hit
GACTTATCCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACT64850.10496205908854088No Hit
GACTTAAGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCA64260.10400712285319408No Hit
GACTTATCCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTT63900.10342444989603335No Hit
GACTTAAGCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGATAAGT63900.10342444989603335No Hit
GACTTAAGGCGGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA63160.10222673326186957No Hit
GACTTATGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA62710.10149839206541862No Hit
GACTTAACGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA62060.10044634367054506No Hit
GACTTAAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCG61910.10020356327172808No Hit
GACTTATCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCC61850.10010645111220129No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTTAA4891700.0144.906561
TTAAGGG403800.0144.566334
ACTTAAG2861500.0144.420532
TTAAGTG459100.0144.41084
CTTAAGG918450.0144.363593
TAAGTGC374450.0144.262945
CTTAAGC993400.0144.25683
AGTGCGT346200.0144.22317
ACTTAAC1371000.0144.187292
GACTTAT1280750.0143.977571
AAGTGCG356250.0143.961826
TTAACCC424200.0143.834294
TAACCCC201150.0143.753945
TTAAGCG468350.0143.60174
AGGGCGC153150.0143.462277
TTAACGC201750.0143.434224
TAAGGGG100800.0143.181375
TAAGGCG207250.0143.090475
CTTAACG422450.0142.988723
TAACGCG99500.0142.939325
Produced by FastQC (version 0.11.2)