FastQCFastQC Report
Thu 6 Oct 2016
HG5MKAFXX_n01_gln3_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5MKAFXX_n01_gln3_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5841096
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGAGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC2641614.522456059616209No Hit
TTCGAGAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT1720742.9459197383504736No Hit
TTCGAGAGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT1370852.346905443772881No Hit
TTCGAGAGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG889291.5224711252819676No Hit
TTCGAGACCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG859991.4723093063356603No Hit
TTCGAGAGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC749601.2833208014386341No Hit
TTCGAGAGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG730211.2501249765454976No Hit
TTCGAGAGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT698231.195374977572702No Hit
TTCGAGAGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA694171.188424227234067No Hit
TTCGAGACGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC690981.182962923396568No Hit
TTCGAGAGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG554000.9484521398038999No Hit
TTCGAGAGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA473610.8108238590839801No Hit
TTCGAGACCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG467030.7995588499144681No Hit
TTCGAGACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCAC456530.7815827714524808No Hit
TTCGAGAGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA449730.7699411206390033No Hit
TTCGAGAGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTC390270.6681451563199783No Hit
TTCGAGTCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC357250.6116146695757098No Hit
TTCGAGATGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA287810.492732870680434No Hit
TTCGAGACGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC287090.4915002253001834No Hit
TTCGAGAGCCGACTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGT277480.4750478334887836No Hit
TTCGAGTGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC263030.45030932551014397No Hit
TTCGAGAGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGAT260840.44656002914521525No Hit
TTCGAGTCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG254760.4361510237119883No Hit
TTCGAGAGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGC229100.3922209119658365No Hit
TTCGAGTCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCAC219480.3757514000797111No Hit
TTCGAGACCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACT216270.3702558560927607No Hit
TTCGAGAACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCT212850.36440079053657054No Hit
TTCGAGACCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGC212150.36320238530577137No Hit
TTCGAGACGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT209500.3586655655034603No Hit
TTCGAGACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGA204500.35010552814060925No Hit
TTCGAGTGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT185290.31721786459253537No Hit
TTCGAGACTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT185170.31701242369582694No Hit
TTCGAGACCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTT177070.3031451631680082No Hit
TTCGAGAGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTT167410.2866071709829799No Hit
TTCGAGAGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTT164320.2813170678927379No Hit
TTCGAGAGTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAA154830.2650701169780466No Hit
TTCGAGTGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT152470.2610297793427809No Hit
TTCGAGACACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCC151400.25919793134713076No Hit
TTCGAGAGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGAT150790.2581536067888629No Hit
TTCGAGAGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCT145350.24884028613808093No Hit
TTCGAGTCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC144450.24729947941276773No Hit
TTCGAGACGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT143120.24502250947424936No Hit
TTCGAGTCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG137180.23485318508718228No Hit
TTCGAGAGAATTACCGCGGTTATACCATGTAGTAAAGGAACTATCAAATA131630.22535154361441756No Hit
TTCGAGACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT130640.22365665621657305No Hit
TTCGAGAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCA117670.20145191929733736No Hit
TTCGAGAGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGG111610.19107715401356185No Hit
TTCGAGAGAATTACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCA110360.18893714467284908No Hit
TTCGAGAGCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCA108780.18623217286618812No Hit
TTCGAGTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG108110.18508512785956607No Hit
TTCGAGAGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC107460.18397232300239544No Hit
TTCGAGATGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC107060.18328752001336737No Hit
TTCGAGAGCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAAC104410.1787507002110563No Hit
TTCGAGACCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCAT103210.17669629124397204No Hit
TTCGAGACCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAA102740.17589164773186403No Hit
TTCGAGAGCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGACTCGA97380.16671528767888766No Hit
TTCGAGAGGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCG96520.16524296125247728No Hit
TTCGAGACCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC95720.1638733552744211No Hit
TTCGAGACCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTTC95500.16349671363045565No Hit
TTCGAGATGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG94740.1621955879513023No Hit
TTCGAGAACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCA91930.15738484695337998No Hit
TTCGAGAGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTT91410.15649460306764346No Hit
TTCGAGAGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGA88270.15111889960377298No Hit
TTCGAGAGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCA86510.1481057664520494No Hit
TTCGAGAGGGAGTTGCTCTAGCTCTTCTTTTCCCCTCTGATCACCTACGG86150.14748944376192413No Hit
TTCGAGAGCCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACA85620.1465820798014619No Hit
TTCGAGAGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTT85130.14574319613990253No Hit
TTCGAGAGCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAA83930.14368878717281824No Hit
TTCGAGAGTCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCA83530.14300398418379015No Hit
TTCGAGAGCTGCGGTTATCAGTACGACCTGGCATGAAAACTATTCCTTCC83330.14266158268927612No Hit
TTCGAGAGAGCTGGAATTACCGCGTTCTAGCATGGATTCTGACTTAGAGG82820.14178845887826533No Hit
TTCGAGAGCGGTTATCAGTACGACCTGGCATGAAAACTATTCCTTCCTGT81770.13999085103206657No Hit
TTCGAGACCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGG81570.13964844953755254No Hit
TTCGAGACCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTG81500.13952860901447262No Hit
TTCGAGACCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACG81120.13887804617489594No Hit
TTCGAGAGCCCGCTGCGTCTGACCAAGGCCCTCACTACCCGACCCTTAGA80700.13815900303641646No Hit
TTCGAGAACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGG80120.13716603870232572No Hit
TTCGAGTGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC80060.1370633182539715No Hit
TTCGAGTCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGA79600.13627579481658922No Hit
TTCGAGAGCCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCT78040.1336050631593797No Hit
TTCGAGAGCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGG77570.13280041964727168No Hit
TTCGAGACCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGT77080.13196153598571228No Hit
TTCGAGACCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCC77000.13182457538790665No Hit
TTCGAGAAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC76760.1314136935944898No Hit
TTCGAGATTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTAC75570.12937640470213124No Hit
TTCGAGACGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCT74200.12703095446471005No Hit
TTCGAGTACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCT72350.12386374064045513No Hit
TTCGAGAATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC72190.12358981944484392No Hit
TTCGAGAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCG71850.12300773690417004No Hit
TTCGAGTGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG71110.12174085137446808No Hit
TTCGAGACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTA69740.11939540113704689No Hit
TTCGAGACCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTA68520.11730675202051122No Hit
TTCGAGATCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCAT68290.11691299030182006No Hit
TTCGAGTGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA66310.11352321550613105No Hit
TTCGAGACTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTACTCGA66280.11347185528195393No Hit
TTCGAGTCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT65860.11275281214347445No Hit
TTCGAGAGCGCCCGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT64940.11117776526870984No Hit
TTCGAGTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT64740.11083536377419581No Hit
TTCGAGTCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGC64630.11064704295221307No Hit
TTCGAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCA64280.11004784033681352No Hit
TTCGAGACCGGGGTGATAAGCTGTTAAGAAGAAAAGATAACTCCTCCCAG63080.10799343136972923No Hit
TTCGAGAGGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC62860.1076167897257638No Hit
TTCGAGACCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTG62500.10700046703563852No Hit
TTCGAGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT61600.10545966031032532No Hit
TTCGAGTGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG60990.1044153357520575No Hit
TTCGAGACGGGGTGATAAGCTGTTAAGAAGAAAAGATAACTCCTCCCAGG59690.1021897260377162No Hit
TTCGAGACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTT59560.10196716506628209No Hit
TTCGAGAGCCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCC58470.10010107692118055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGAGA4799650.0144.857731
TCGAGAG2696200.0144.752412
TTCGAGT978900.0144.667681
CGAGACC664700.0144.633393
AGAGTGC299350.0144.594925
CGAGAGG819500.0144.548933
TCGAGTG287300.0144.529452
TCGAGAC1394900.0144.52112
GAGAGTG370600.0144.473834
CGAGACG398000.0144.472413
CGAGAGC1117450.0144.450423
TCGAGTC534450.0144.3192
CGAGAGT616550.0144.265183
GAGAGGG430350.0143.803254
GAGAGCC385200.0143.627434
GAGAGCG571500.0143.612494
CGAGTCG169200.0143.01163
GAGACCC477400.0143.008394
AGACGCG110000.0142.939455
GAGGGCG233150.0142.868136
Produced by FastQC (version 0.11.2)