FastQCFastQC Report
Thu 6 Oct 2016
HG5MKAFXX_n01_gln3_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5MKAFXX_n01_gln3_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6162766
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTCCAGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC2314033.754856179838728No Hit
AGTTCCAGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT1358722.204724307234771No Hit
AGTTCCTGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC613490.9954783290490018No Hit
AGTTCCAGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT605170.9819778975868952No Hit
AGTTCCAGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTC586990.9524781567237828No Hit
AGTTCCAGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA510360.828134639543348No Hit
AGTTCCAGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG478910.7771023595573806No Hit
AGTTCCTGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT410280.665740026475125No Hit
AGTTCCAGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCA404360.6561339502424722No Hit
AGTTCCAGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG310450.5037510754099701No Hit
AGTTCCAGAATTACCGCGGTTATACCATGTAGTAAAGGAACTATCAAATA295710.47983324370907476No Hit
AGTTCCAGCCGACTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGT273630.44400517559809993No Hit
AGTTCCAGGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCG271620.4407436530934324No Hit
AGTTCCAGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA214500.34805799863243225No Hit
AGTTCCAGAATTACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCA212550.344893835008501No Hit
AGTTCCTCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC189630.30770274256721736No Hit
AGTTCCTGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT175180.284255478789881No Hit
AGTTCCTGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTC174590.2832981164626403No Hit
AGTTCCAGAGCTGGAATTACCGCGTTCTAGCATGGATTCTGACTTAGAGG171190.2777811132209141No Hit
AGTTCCTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG157720.25592404449560474No Hit
AGTTCCATGGAGCTGGAATTACCGCGTTCTAGCATGGATTCTGACTTAGA155860.25290591919277805No Hit
AGTTCCTGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA151940.24654513898467018No Hit
AGTTCCTGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG151880.24644778010393387No Hit
AGTTCCTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT144800.23495943217704518No Hit
AGTTCCTGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCA136660.22175107735714775No Hit
AGTTCCTAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA124400.20185741272668797No Hit
AGTTCCTAGCTGGAATTACCGCGGTTATACCATGTAGTAAAGGAACTATC120820.19604833284275278No Hit
AGTTCCTACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCA120020.19475021443293483No Hit
AGTTCCACGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT107610.1746131526006342No Hit
AGTTCCAGAGCTGGAATTACCGCGGTTATACCATGTAGTAAAGGAACTAT97510.15822440767668283No Hit
AGTTCCATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCA91200.1479854987192439No Hit
AGTTCCTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT87980.14276057211972676No Hit
AGTTCCTGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG87830.1425171749178859No Hit
AGTTCCAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCG81480.1322133600399561No Hit
AGTTCCTGGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCG80360.13039599426621098No Hit
AGTTCCAGGAATTACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTC78440.12728051008264796No Hit
AGTTCCTGAATTACCGCGGTTATACCATGTAGTAAAGGAACTATCAAATA76280.12377559037613954No Hit
AGTTCCTAGCAGACAAATCACTCCACCAACTAAGAACGGCCATGCACCAC74500.12088727691429466No Hit
AGTTCCAGGAGCTGGAATTACCGCGTTCTAGCATGGATTCTGACTTAGAG72820.118161228253677No Hit
AGTTCCTCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT69730.11314724589575524No Hit
AGTTCCAGCTGGAATTACCGCGGTTATACCATGTAGTAAAGGAACTATCA66660.10816571649807895No Hit
AGTTCCAGGAATTACCGCGGTTATACCATGTAGTAAAGGAACTATCAAAT65400.10612118000261571No Hit
AGTTCCAGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAAT62780.10186984221046198No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCCA4169500.0144.41631
GTTCCAG2402500.0143.879992
AGTTCCT1981400.0143.331131
GTTCCAC794350.0142.453462
TCCAGGG446950.0142.370064
GTTCCTG682550.0142.177492
TCCAGTG362850.0141.924674
GTTCCAT625150.0141.746112
CAGTGCG249650.0141.14736
GTTCCTA343700.0141.016162
CCAGTGC268200.0140.548635
AGTGCGT242700.0140.223137
TCCAGGC256350.0139.891274
TTCCAGT558050.0139.733373
TTCCACG238750.0139.667863
TTCCACC314900.0139.226263
TTCCAGG876400.0138.9683
TCCTAGC77600.0138.815874
TCCTGGG137150.0138.585864
CCAGGGG156000.0138.336335
Produced by FastQC (version 0.11.2)