FastQCFastQC Report
Thu 6 Oct 2016
HG5MKAFXX_n01_gln3_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5MKAFXX_n01_gln3_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6146590
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGATCAGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC2305283.750502311037502No Hit
CAGATCAGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT994171.6174334061650444No Hit
CAGATCAGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT623681.0146764303459315No Hit
CAGATCTGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC543800.8847181933397217No Hit
CAGATCACCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG482310.7846789846077254No Hit
CAGATCAGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA476600.7753892808858245No Hit
CAGATCAGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG427460.6954425136539122No Hit
CAGATCAGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG415690.6762936847910792No Hit
CAGATCAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT399420.6498237233978515No Hit
CAGATCTCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG351440.5717641814404409No Hit
CAGATCAGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA339980.5531196972630352No Hit
CAGATCACGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT330480.5376639730322016No Hit
CAGATCACCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTT292700.47619899814368616No Hit
CAGATCACCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG274700.44691446802210655No Hit
CAGATCACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCAC270830.4406182940459669No Hit
CAGATCTCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCAC269590.4386009153042581No Hit
CAGATCATGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA262410.426919641622428No Hit
CAGATCTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT253380.4122285690114356No Hit
CAGATCAGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG241040.3921523966947527No Hit
CAGATCTGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT239180.3891263285821895No Hit
CAGATCAGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGAT235860.3837249596930981No Hit
CAGATCAGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTC224420.3651130138824942No Hit
CAGATCTCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG206650.33620267497913475No Hit
CAGATCAGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC205790.3348035252066593No Hit
CAGATCAGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGC204390.33252583953053644No Hit
CAGATCACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGA199620.32476543904831784No Hit
CAGATCAGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTT197310.3210072576827151No Hit
CAGATCTCCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTT188670.3069506832243569No Hit
CAGATCAGCCGACTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGT185250.3013866225012568No Hit
CAGATCTCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGA180610.2938377214032496No Hit
CAGATCAGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTT178570.2905188079894706No Hit
CAGATCACGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC176760.28757408579391175No Hit
CAGATCTCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC153810.2502363098888977No Hit
CAGATCAGTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAA153350.24948792745245738No Hit
CAGATCTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC145370.2365051190985571No Hit
CAGATCACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATG138410.2251817674515463No Hit
CAGATCAGCCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCT133530.21724240595191804No Hit
CAGATCAGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA133090.21652656188227948No Hit
CAGATCACGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT131620.21413499192235044No Hit
CAGATCTCGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATG127810.20793643304661608No Hit
CAGATCAATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC127260.2070416279595678No Hit
CAGATCTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT124160.2019981811052958No Hit
CAGATCAGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTT120730.19641785119879476No Hit
CAGATCAGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAG120720.1964015820153939No Hit
CAGATCAGAGCTGGAATTACCGCGTTCTAGCATGGATTCTGACTTAGAGG113350.18441119384894714No Hit
CAGATCAGCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGCA112250.18262158367485062No Hit
CAGATCATCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCAT111970.18216604653962604No Hit
CAGATCATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCA110960.1805228590161374No Hit
CAGATCAACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGG110520.17980701494649878No Hit
CAGATCAGGCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGC110050.17904236332665754No Hit
CAGATCAGTTCTGCTTACCAAAAATGGCCCACTAAAAGCTCTTCATTCAA109270.1777733670213891No Hit
CAGATCACCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTTC108180.17600002603069345No Hit
CAGATCAGAATTACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCA108170.17598375684729256No Hit
CAGATCAGCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGG105660.1719001918136723No Hit
CAGATCATGGAGCTGGAATTACCGCGTTCTAGCATGGATTCTGACTTAGA104180.16949235267034243No Hit
CAGATCACACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCC102230.1663198619071713No Hit
CAGATCTGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG101860.16571790212133883No Hit
CAGATCAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATC100850.1640747145978502No Hit
CAGATCACCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGC100190.16300094849339225No Hit
CAGATCAGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGAC99310.16156926035411504No Hit
CAGATCTGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA98540.16031653323224748No Hit
CAGATCTCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT94830.15428066619052191No Hit
CAGATCAATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC91310.148553913633413No Hit
CAGATCACCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGT90860.1478218003803735No Hit
CAGATCTGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG90240.1468131110095191No Hit
CAGATCTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGT89690.14591830592247085No Hit
CAGATCAACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCT88730.1443564643159866No Hit
CAGATCAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCG87260.14196489435605758No Hit
CAGATCACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT86580.1408585898847979No Hit
CAGATCTATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC86040.13998005398115052No Hit
CAGATCTGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT85680.13939436337871894No Hit
CAGATCTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCT85650.1393455558285163No Hit
CAGATCTCGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT85140.13851582747507155No Hit
CAGATCAGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGA85140.13851582747507155No Hit
CAGATCTACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGG84360.13724683116980307No Hit
CAGATCAGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC82980.13500168386048197No Hit
CAGATCACGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCT82920.1349040687600767No Hit
CAGATCAGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCA82060.13350491898760125No Hit
CAGATCACCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCC81540.1326589214507556No Hit
CAGATCACCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGC81370.1323823453329407No Hit
CAGATCAGCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCA80780.13142246351228892No Hit
CAGATCAGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT80010.13016973639042134No Hit
CAGATCTCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTTC78780.1281686268321134No Hit
CAGATCTGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA77800.1265742468588274No Hit
CAGATCTCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCC77270.1257119801385809No Hit
CAGATCTCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGC75960.12358071711306594No Hit
CAGATCTCCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGC75200.12234425917459923No Hit
CAGATCAACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCA74040.12045703390009745No Hit
CAGATCAGCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGT73240.11915549922802725No Hit
CAGATCACTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGAGATCTG73220.11912296086122551No Hit
CAGATCAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCCGGTGAGTTG73080.1188951922936132No Hit
CAGATCAGCGGTTATCAGTACGACCTGGCATGAAAACTATTCCTTCCTGT71720.11668258335109387No Hit
CAGATCAGCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGAGATCT70690.11500685746080347No Hit
CAGATCAGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAA70670.11497431909400171No Hit
CAGATCAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACT70630.11490924236039819No Hit
CAGATCAGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTC70510.11471401215958767No Hit
CAGATCAGATCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGG70220.11424220584096223TruSeq Adapter, Index 19 (97% over 36bp)
CAGATCACGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC69730.11344501585431922No Hit
CAGATCAGAATTACCGCGGTTATACCATGTAGTAAAGGAACTATCAAATA69030.11230617301625781No Hit
CAGATCAGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCT68770.11188317424783499No Hit
CAGATCACGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAAC68210.11097209997738583No Hit
CAGATCAGCAGACAAATCACTCCACCAACTAAGAACGGCCATGCACCACC68110.11080940814337706No Hit
CAGATCTACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCA68060.11072806222637266No Hit
CAGATCAGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGT67290.1094753351045051No Hit
CAGATCAGCTGGAATTACCGCGTTCTAGCATGGATTCTGACTTAGAGGCG66340.10792976268142174No Hit
CAGATCAGCTGCGGTTATCAGTACGACCTGGCATGAAAACTATTCCTTCC65210.10609134495712257No Hit
CAGATCTACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCT64290.10459458008424184No Hit
CAGATCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCA63730.10368350581379268No Hit
CAGATCTCGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAAC62880.1023006252247181No Hit
CAGATCAGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAAT62600.10184508808949352No Hit
CAGATCAGCCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACA62580.10181254972269178No Hit
CAGATCAGCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAAC62430.10156851197167861No Hit
CAGATCTCCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCC62380.10148716605467421No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATCA4277300.0144.771031
TCAGTGC266800.0143.901345
GATCAGT602000.0143.723753
TCAGGCG136200.0143.710775
CAGTGCG251700.0143.692126
AGTGCGT244300.0143.669687
GATCAGG702550.0143.533253
ATCACGC136800.0143.504414
GATCACT204650.0143.500413
ATCAGGG327200.0142.949864
TCAGCCG91850.0142.857185
AGATCAC1098200.0142.804382
AGGGCGC105950.0142.717477
ATCAGTG370900.0142.681824
ATCACCC331000.0142.425724
GATCTCC402300.0142.231163
GATCACG323900.0142.145723
TCAGGGG80200.0141.73415
AGATCAG2529250.0141.670262
ATCTCGC125100.0141.56924
Produced by FastQC (version 0.11.2)