FastQCFastQC Report
Thu 6 Oct 2016
HG5MKAFXX_n01_gln3_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5MKAFXX_n01_gln3_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6920928
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAATAGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC3626675.240149875854798No Hit
GCCAATAGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT1481862.1411290509018444No Hit
GCCAATAGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG1452172.098230179536617No Hit
GCCAATACCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG884701.278296783321543No Hit
GCCAATAGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT756561.0931482020908179No Hit
GCCAATAGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA601680.8693631836655431No Hit
GCCAATAGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG594240.8586131801978No Hit
GCCAATAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT569010.8221585313414617No Hit
GCCAATAGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA546140.7891138298216656No Hit
GCCAATACGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT540090.7803722275394283No Hit
GCCAATACCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG537760.7770056269910625No Hit
GCCAATAGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTC471190.680819104027668No Hit
GCCAATACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCAC446600.6452891866524259No Hit
GCCAATACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATG410830.5936053662167847No Hit
GCCAATACGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC367360.5307958701492055No Hit
GCCAATAGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC337280.48733349053768515No Hit
GCCAATAATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC333490.4818573462980687No Hit
GCCAATAGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG327010.4724944400519699No Hit
GCCAATACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGA316760.4576842874250389No Hit
GCCAATATGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA313980.45366748505402743No Hit
GCCAATAGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA289520.4183254037608829No Hit
GCCAATAGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGAT266390.3849050300768914No Hit
GCCAATTGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC264450.3821019377748186No Hit
GCCAATAGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTT254410.36759521266512235No Hit
GCCAATACCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTT232900.33651556554265555No Hit
GCCAATAGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGC225650.3260400917333629No Hit
GCCAATAGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAA221640.3202460710471197No Hit
GCCAATAGCCGACTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGT209950.3033552725877223No Hit
GCCAATTCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG209080.30209821573060724No Hit
GCCAATAGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTC193890.2801502919839651No Hit
GCCAATAGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAG191970.27737609754067666No Hit
GCCAATACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT189040.2731425612287832No Hit
GCCAATACACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCC183950.2657880561681902No Hit
GCCAATAGTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAA181310.26197353880866847No Hit
GCCAATACGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT181250.26188684523231565No Hit
GCCAATACCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGC179410.25922824222416413No Hit
GCCAATATCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCAT168720.24378233670397956No Hit
GCCAATAGGCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGC164830.23816170317044189No Hit
GCCAATTCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCAC163290.2359365680440542No Hit
GCCAATAGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGA161430.23324906717711844No Hit
GCCAATAGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTT161310.2330756800244129No Hit
GCCAATAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCG159880.23100948312133862No Hit
GCCAATACCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGT158580.22913112230036203No Hit
GCCAATACGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC155260.22433407774217562No Hit
GCCAATAACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGG151470.2188579335025592No Hit
GCCAATTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC150980.21814993596234494No Hit
GCCAATATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCA150710.2177598148687575No Hit
GCCAATAGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGT147020.21242815992306235No Hit
GCCAATAATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC142530.20594059062599696No Hit
GCCAATTCGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATG141710.2047557784158425No Hit
GCCAATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT134200.19390463244235456No Hit
GCCAATAACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCT130460.18850073284969876No Hit
GCCAATATTACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC128680.18592882341790004No Hit
GCCAATAGCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGAATTGG128240.1852930705246464No Hit
GCCAATACCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGC128060.1850329897955881No Hit
GCCAATTCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG125640.18153634888269318No Hit
GCCAATACCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCAT124720.18020704737861742No Hit
GCCAATTCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC124540.17994696664955914No Hit
GCCAATTGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT123860.17896443945089446No Hit
GCCAATAGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTT123220.1780397079697983No Hit
GCCAATAGCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGG122280.17668150860693826No Hit
GCCAATAGCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGCA117030.16909582067607118No Hit
GCCAATAACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCA116040.16766537666625053No Hit
GCCAATAGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC115970.16756423416050564No Hit
GCCAATTGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG114740.16578701584527394No Hit
GCCAATACTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT114040.164775590787825No Hit
GCCAATACCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTTC113310.16372081894219964No Hit
GCCAATAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCA112680.16281053639049559No Hit
GCCAATAGCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCA107210.15490697201300171No Hit
GCCAATAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATC105700.15272518367479043No Hit
GCCAATACCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAA104760.15136698431193044No Hit
GCCAATACGGACGGGAAACGGTGCTTTCTGGTAGATATGGCAATTGGCAG98270.1419896291364395No Hit
GCCAATACCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC98140.14180179305434185No Hit
GCCAATTCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGA96300.13914319004619036No Hit
GCCAATAGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGAC95680.13824735642387842No Hit
GCCAATAGGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA92610.13381153510049518No Hit
GCCAATACGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTT88830.1283498397902709No Hit
GCCAATACTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTAATTGG87980.12712168079194003No Hit
GCCAATAGCCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCT87910.12702053828619514No Hit
GCCAATAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACT87410.12629809181658877No Hit
GCCAATACGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCT87100.12585017500543277No Hit
GCCAATAGCTGCGGTTATCAGTACGACCTGGCATGAAAACTATTCCTTCC85680.12379842703175066No Hit
GCCAATAGGCCCAGTGAAATGCGAGATTCCCCTACCCACAAGGAGCAGAG84690.12236798302193001No Hit
GCCAATACCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACG83880.12119761974116766No Hit
GCCAATACCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCC83860.1211687218823834No Hit
GCCAATAGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTT83630.12083639650636448No Hit
GCCAATAGCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTAATTG82480.11917476962626976No Hit
GCCAATAGCGGTTATCAGTACGACCTGGCATGAAAACTATTCCTTCCTGT82170.1187268528151138No Hit
GCCAATAGGACGGGAAACGGTGCTTTCTGGTAGATATGAATTGGCAGATC81380.1175853873931357No Hit
GCCAATACGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCA80590.11644392197115762No Hit
GCCAATAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCCGGTGAGTTG79740.11521576297282676No Hit
GCCAATAGTTCTGCTTACCAAAAATGGCCCACTAAAAGCTCTTCATTCAA79030.1141898889859857No Hit
GCCAATACCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCT77220.11157463276601058No Hit
GCCAATACGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA75990.10979741445077885No Hit
GCCAATAGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT75530.10913276369874099No Hit
GCCAATACTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA75340.10885823404029056No Hit
GCCAATAGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAG75200.10865594902880077No Hit
GCCAATAGCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGT74850.1081502365000763No Hit
GCCAATAGCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAA74210.10722550501898011No Hit
GCCAATAGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT73780.10660420105511861No Hit
GCCAATACGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAAC72610.10491367631623967No Hit
GCCAATTCGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT72140.10423457663480966No Hit
GCCAATAGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGAT71940.10394559804696711No Hit
GCCAATAGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTA69930.10104136323914943No Hit
GCCAATAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGA69570.10052120178103284No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATAG3160950.0144.582812
CCAATAC1728950.0144.396742
TAGGCGC173700.0144.311816
TAGTGCG395450.0144.209646
ATAGCCC176650.0143.78955
CAATAGG1154400.0143.761323
ATAGTGC419200.0143.69995
TAGGGCG241950.0143.691226
AATAGTG539300.0143.595084
CAATAGT837800.0143.543243
AGTGCGT388550.0143.39277
AGGGCGC158900.0143.244967
ATACGCG82600.0143.136585
AGGCGCA155050.0143.061667
TACGCGC59550.0142.910146
AATAGCC375150.0142.892674
ATAGCGC190350.0142.846945
AATAGCG407650.0142.792314
AATAGGG485500.0142.69514
AATACCC525550.0142.45544
Produced by FastQC (version 0.11.2)