FastQCFastQC Report
Thu 6 Oct 2016
HG5MKAFXX_n01_gln3_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5MKAFXX_n01_gln3_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7558195
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGAAGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC3261064.314601568231568No Hit
ACTTGAAGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT1364701.8055898266715795No Hit
ACTTGAACCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG947251.2532754182711614No Hit
ACTTGAAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT871701.1533176902686422No Hit
ACTTGATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC830511.098820551732259No Hit
ACTTGAAGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT712410.9425663137825896No Hit
ACTTGAAGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG700560.9268879672990707No Hit
ACTTGATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG698480.9241359874943686No Hit
ACTTGAAGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA650950.8612506028224992No Hit
ACTTGAAGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG634940.8400682967295764No Hit
ACTTGAACGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT507400.6713243042816439No Hit
ACTTGAAGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA507090.6709141534453663No Hit
ACTTGAAGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC490270.6486601629092661No Hit
ACTTGATCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT488650.6465167940229116No Hit
ACTTGAACCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG459660.6081610754948767No Hit
ACTTGAACGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC451180.5969414655218607No Hit
ACTTGAAGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG428550.5670004544735879No Hit
ACTTGAAGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTC406040.537218211490971No Hit
ACTTGAAGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA406000.5371652888024191No Hit
ACTTGATGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT363900.4814641591014786No Hit
ACTTGATCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC356600.47180576844074545No Hit
ACTTGATCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG343990.45512189087473925No Hit
ACTTGAACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCAC312990.41410680724696836No Hit
ACTTGATCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCAC298680.3951737154175038No Hit
ACTTGAACCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTT297290.3933346519903231No Hit
ACTTGAATGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA284050.3758172420796235No Hit
ACTTGAAGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGAT242060.3202616497722009No Hit
ACTTGAACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGA233310.3086848116514591No Hit
ACTTGAACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATG221390.2929138504629743No Hit
ACTTGAACGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC220690.2919877034133149No Hit
ACTTGAAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCG217380.2876083509356401No Hit
ACTTGATCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGA192460.25463751596776746No Hit
ACTTGAACGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT192060.2541082890822478No Hit
ACTTGATCCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTT185440.24534958412689803No Hit
ACTTGATCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC182230.24110253837060303No Hit
ACTTGATCGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATG177560.23492381448216143No Hit
ACTTGATGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG174760.23121922628352404No Hit
ACTTGAAGGGAGTTGCTCTAGCTCTTCTTTTCCCCTCTGATCACCTACGG174060.2302930792338647No Hit
ACTTGATGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG170270.22527865449356627No Hit
ACTTGAAGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGC167680.22185191040982669No Hit
ACTTGAACCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGC164350.2174460965878758No Hit
ACTTGAAGGAACTATCAAATAAACGATAACTGATTTAATGAGCCATTCGC164220.21727409785008192No Hit
ACTTGAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCG161950.21427073527475804No Hit
ACTTGATGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA158970.21032799497763688No Hit
ACTTGAACCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCAT157790.20876677566535398No Hit
ACTTGAATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCA156470.2070203269431392No Hit
ACTTGAACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT154170.20397727235140134No Hit
ACTTGAAGTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAA149660.1980102392171676No Hit
ACTTGATCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT149130.1973090135938541No Hit
ACTTGAATCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCAT147380.19499364596970573No Hit
ACTTGATCGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT143770.19021737332789113No Hit
ACTTGAACACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCC143070.18929122627823178No Hit
ACTTGAAGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTT141650.1874124708346371No Hit
ACTTGATCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCAT135860.17975191166674054No Hit
ACTTGAACTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT134220.17758208143611007No Hit
ACTTGATATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC133810.17703962387845246No Hit
ACTTGATGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT132900.1758356327138953No Hit
ACTTGATGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA129640.17152243359691036No Hit
ACTTGATCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGC129520.17136366553125448No Hit
ACTTGATCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT129470.17129751217056455No Hit
ACTTGAAGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGA127640.16887629916931224No Hit
ACTTGAAATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC126970.16798984413606688No Hit
ACTTGAACCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC126490.16735477187344333No Hit
ACTTGAACTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGATCAAGT121040.16014405555823844No Hit
ACTTGAAGTGGGAGTTGCTCTAGCTCTTCTTTTCCCCTCTGATCACCTAC117270.15515609216221599No Hit
ACTTGAAGGTCAGGCTCTTACCAGCTTAACTACAGTTGATCGGACGGGAA116250.15380656360414094No Hit
ACTTGATGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTC115550.1528804165544816No Hit
ACTTGAAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCA114630.15166319471778647No Hit
ACTTGAACCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTTC114050.150895815733783No Hit
ACTTGAAGCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGATCAAG113210.1497844392741918No Hit
ACTTGAAGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC109630.14504785864879113No Hit
ACTTGAAGCCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACA109410.14475678386175536No Hit
ACTTGATGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA108680.14379094479568202No Hit
ACTTGAAACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCT105790.13996728054780275No Hit
ACTTGAACCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAA104900.1387897507275216No Hit
ACTTGATATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC103950.13753283687441248No Hit
ACTTGAAGCCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCT103920.13749314485799852No Hit
ACTTGAAGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTT99880.1321479533142503No Hit
ACTTGAAGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTT99350.1314467276909368No Hit
ACTTGAAGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGAT91760.12140464753820192No Hit
ACTTGAAGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAA90430.11964496814384916No Hit
ACTTGAAGCCGACTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGT88890.1176074446345986No Hit
ACTTGAAGTCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCA87930.11633730010935152No Hit
ACTTGATCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGATCAAGT84690.11205056233664255No Hit
ACTTGAAATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC83950.11107149259843123No Hit
ACTTGATCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAA83740.11079364848353343No Hit
ACTTGAAGCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAA79220.10481338467716167No Hit
ACTTGAACCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCC78000.10319924267632682No Hit
ACTTGAATGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC76560.10129402588845618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAGC944300.0144.53873
TGAAGGG461250.0144.481024
ACTTGAA5276450.0144.390141
TGAAGCG415950.0144.373964
TTGAAGG973150.0144.283723
CTTGAAG2845350.0144.247012
CTTGAAC1505200.0143.791462
TGAAGTG469400.0143.779364
AAGTGCG345000.0143.702216
AGTGCGT333500.0143.473667
TGATCCC368400.0143.44914
TTGAACG484200.0143.367023
TTGATCC513900.0143.319433
TTGAAGT764100.0143.200353
AGGGCGC145850.0143.035137
GAAGGGC275900.0143.013345
GAACGCG87450.0142.911585
TGAAGCC355950.0142.723364
ACTTGAT2304000.0142.687741
TGATCCG68050.0142.640344
Produced by FastQC (version 0.11.2)