FastQCFastQC Report
Thu 6 Oct 2016
HG5MKAFXX_n01_gln3_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5MKAFXX_n01_gln3_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6999718
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCCAGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC2209083.1559557113586574No Hit
GTGGCCAGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA1347161.9245918192704337No Hit
GTGGCCAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT1125981.608607661051488No Hit
GTGGCCAGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT862791.232606799302486No Hit
GTGGCCAGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA756701.0810435506116105No Hit
GTGGCCTCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC642370.9177083991097926No Hit
GTGGCCACGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC636590.9094509235943505No Hit
GTGGCCAGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG617360.8819783882722133No Hit
GTGGCCAGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGAT587160.8388337930185187No Hit
GTGGCCAGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG571520.81649003574144No Hit
GTGGCCAGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC556510.7950463147229645No Hit
GTGGCCAGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT499870.7141287691875586No Hit
GTGGCCAGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA433850.6198106838018332No Hit
GTGGCCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCG368250.52609262258851No Hit
GTGGCCAGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG360070.5144064375164829No Hit
GTGGCCTGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT333620.47661920094495236No Hit
GTGGCCACGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT329070.47011893907726No Hit
GTGGCCAGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGT321290.459004205597997No Hit
GTGGCCATGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA287190.41028795731485185No Hit
GTGGCCAGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGC285140.40735926790193544No Hit
GTGGCCAGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGAT279270.39897321577812134No Hit
GTGGCCACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATG272050.3886585145287281No Hit
GTGGCCTGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA260210.3717435473829089No Hit
GTGGCCAGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTT258390.3691434426358319No Hit
GTGGCCACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGA256260.3661004629043627No Hit
GTGGCCACCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC255130.364486112154804No Hit
GTGGCCACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCAC254400.36344321299800936No Hit
GTGGCCTGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC253040.36150027758261116No Hit
GTGGCCAGGAACTATCAAATAAACGATAACTGATTTAATGAGCCATTCGC239520.34218521374718236No Hit
GTGGCCAATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC237990.33999941140485945No Hit
GTGGCCACCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG234880.33555637527111803No Hit
GTGGCCACGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC234190.3345706212735999No Hit
GTGGCCACAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATT229120.3273274723353141No Hit
GTGGCCACTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT217880.311269682578641No Hit
GTGGCCTCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC201060.28724014310290785No Hit
GTGGCCACCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCAT199610.28516863107913776No Hit
GTGGCCAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAA181220.2588961441018052No Hit
GTGGCCACGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTAT180870.2583961239581366No Hit
GTGGCCTCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC180410.2577389546264578No Hit
GTGGCCTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG178990.255710301472145No Hit
GTGGCCAACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCT174550.24936718879246278No Hit
GTGGCCAGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTC172380.24626706390171718No Hit
GTGGCCTCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCAC146630.20947986761752402No Hit
GTGGCCTGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC146290.20899413376367446No Hit
GTGGCCACCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG145340.20763693623085958No Hit
GTGGCCAGAATTACCGCGGTTATACCATGTAGTAAAGGAACTATCAAATA144700.2067226136824369No Hit
GTGGCCTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC144140.2059225814525671No Hit
GTGGCCTCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCAT142660.2038082105593397No Hit
GTGGCCTCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGA142550.20365106137132954No Hit
GTGGCCACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT137040.19577931568100312No Hit
GTGGCCTCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATT136300.19472213023438945No Hit
GTGGCCTGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT134230.1917648682418349No Hit
GTGGCCAGTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAA131360.187664703063752No Hit
GTGGCCTCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT128950.1842217072173479No Hit
GTGGCCAATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC125380.1791215017519277No Hit
GTGGCCAGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC120380.17197835684237564No Hit
GTGGCCTCGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATG119500.17072116333829448No Hit
GTGGCCATTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTAC119350.17050686899100792No Hit
GTGGCCAGCCGACTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGT116080.16583525222016088No Hit
GTGGCCAGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGA115930.1656209578728743No Hit
GTGGCCATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCA114200.1631494297341693No Hit
GTGGCCATGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC111870.15982072420631802No Hit
GTGGCCAAGCTGGAATTACCGCGGTTATACCATGTAGTAAAGGAACTATC111520.1593207040626494No Hit
GTGGCCAGCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGG108940.1556348412893205No Hit
GTGGCCTGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGAT104970.14996318423113617No Hit
GTGGCCAGGCGGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA104190.14884885362524605No Hit
GTGGCCAGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGG101000.14429152717295182No Hit
GTGGCCTGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG100560.14366293042091124No Hit
GTGGCCAGCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGCA97820.1397484870104767No Hit
GTGGCCTATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAA95430.13633406374371082No Hit
GTGGCCTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGT94050.13436255574867445No Hit
GTGGCCTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTAT93800.13400539850319684No Hit
GTGGCCAGGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCG93510.1335910960984428No Hit
GTGGCCAACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGG92530.13219103969617063No Hit
GTGGCCTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT90340.1290623422257868No Hit
GTGGCCAGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGC89960.12851946321266086No Hit
GTGGCCACTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGC89690.12813373338754502No Hit
GTGGCCAGGCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGC88500.12643366489907165No Hit
GTGGCCAGCGGTTATCAGTACGACCTGGCATGAAAACTATTCCTTCCTGT87040.12434786658548246No Hit
GTGGCCATGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG86880.12411928594837678No Hit
GTGGCCAGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTA86810.12401928191964305No Hit
GTGGCCAGGGGTACAAAGGTTATGGGACTCATCAACCAAGTTGGATTCAG86440.1234906891963362No Hit
GTGGCCTCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG85950.1227906609952001No Hit
GTGGCCATCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCAT85690.12241921745990339No Hit
GTGGCCATTACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC85480.1221192053737022No Hit
GTGGCCAAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTC84970.12139060459292789No Hit
GTGGCCTGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA84340.12049056833432434No Hit
GTGGCCACCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAA84090.12013341108884673No Hit
GTGGCCAGGGAGTTGCTCTAGCTCTTCTTTTCCCCTCTGATCACCTACGG83450.11921908854042404No Hit
GTGGCCAGCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAAC83370.11910479822187121No Hit
GTGGCCAGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAA82860.11837619744109693No Hit
GTGGCCAGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCT82840.1183476248614587No Hit
GTGGCCAGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTT81840.11691899587954828No Hit
GTGGCCTCGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT81700.11671898782208083No Hit
GTGGCCACCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGT81200.11600467333112562No Hit
GTGGCCACACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCC80190.11456175805939611No Hit
GTGGCCACCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAA78960.11280454441164631No Hit
GTGGCCAGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTT78240.1117759315446708No Hit
GTGGCCAGCGGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGAT76940.10991871386818726No Hit
GTGGCCTGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA76620.10946155259397594No Hit
GTGGCCAGAGCTGGAATTACCGCGGTTATACCATGTAGTAAAGGAACTAT76210.10887581471139265No Hit
GTGGCCAGGGGGTATGAACAACAATTGTTGTAGTCGCAACTACGGTAATT76160.10880438326229713No Hit
GTGGCCAGGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC74840.1069185930061754No Hit
GTGGCCATGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC74240.10606141561702914No Hit
GTGGCCACCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGC71440.10206125446768No Hit
GTGGCCAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCCGGTGAGTTG70820.10117550449889554No Hit
GTGGCCAGCCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACA70040.10006117389300541No Hit
GTGGCCACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAG70000.10000402873372898No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGCCA5077850.0144.7741
TGGCCAG2871850.0144.594642
GCCAGCG498300.0143.900774
GGCCAGC1060050.0143.883153
CCAGTGC270700.0143.718695
CAGTGCG243000.0143.694536
GGCCAGT717550.0143.674093
GCCAGTG364400.0143.50524
GTGGCCG193000.0143.480031
AGTGCGT231050.0143.437917
GGCCTCG287550.0142.60763
GGCCTCC269450.0142.582953
GCCACGC230600.0142.556784
GCCACCC322250.0142.36794
GCCTCGG61500.0141.794834
GGCCACG453400.0141.635823
TGGCCTA199100.0141.51952
GCCTGGG60000.0141.473654
GCCAGCC331350.0141.389474
GCCTGCG118950.0141.320834
Produced by FastQC (version 0.11.2)