FastQCFastQC Report
Thu 6 Oct 2016
HG5MKAFXX_n01_gln3_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5MKAFXX_n01_gln3_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7241995
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTAGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC4360396.020979025807115No Hit
TAGCTTAGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT1375431.899241852555822No Hit
TAGCTTAGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG1284881.7742072453792086No Hit
TAGCTTACCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG1082391.494601970865763No Hit
TAGCTTAGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA1022891.4124422897281756No Hit
TAGCTTACGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT1018071.4057866651385427No Hit
TAGCTTAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT819521.1316218804348803No Hit
TAGCTTAGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT792021.0936489185645668No Hit
TAGCTTAGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA606920.8380563643029304No Hit
TAGCTTACCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG574020.7926268935562645No Hit
TAGCTTAGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG512860.7081750263566876No Hit
TAGCTTAGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA502910.6944357183345197No Hit
TAGCTTACGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC469450.6482329800006766No Hit
TAGCTTAGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC469390.6481501299020505No Hit
TAGCTTTGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC427220.5899203189176463No Hit
TAGCTTTCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG414370.5721765894618817No Hit
TAGCTTAGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTC398190.5498346795323664No Hit
TAGCTTTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT368960.5094728731516661No Hit
TAGCTTACCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTT366260.5057446187134899No Hit
TAGCTTAGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG358280.4947255555962135No Hit
TAGCTTAGGAACTATCAAATAAACGATAACTGATTTAATGAGCCATTCGC341030.47090615224119875No Hit
TAGCTTATGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA320250.4422124014170128No Hit
TAGCTTACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCAC291610.4026652876728029No Hit
TAGCTTAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCG285410.39410411081476854No Hit
TAGCTTACCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGC278200.384148290629861No Hit
TAGCTTATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCA259360.3581333596612536No Hit
TAGCTTACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGA254030.3507735092332983No Hit
TAGCTTAGGGAGTTGCTCTAGCTCTTCTTTTCCCCTCTGATCACCTACGG245600.3391330703763259No Hit
TAGCTTAGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGC229710.31719160259017026No Hit
TAGCTTACGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT226740.31309052270817644No Hit
TAGCTTTCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG222410.30711150725732345No Hit
TAGCTTATCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCAT221560.30593779752678646No Hit
TAGCTTACACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCC217260.30000020712524655No Hit
TAGCTTAATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC216500.2989507725426488No Hit
TAGCTTACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATG215660.2977908711618829No Hit
TAGCTTAGTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAA209660.2895058612992691No Hit
TAGCTTAGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGAT206950.28576379851132183No Hit
TAGCTTAACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCT206230.28476959732780815No Hit
TAGCTTTCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC202070.27902532382306255No Hit
TAGCTTACGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC201460.27818301448703014No Hit
TAGCTTAGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTT193270.26687397602456225No Hit
TAGCTTAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCG192740.2661421334866981No Hit
TAGCTTACTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT172380.2380283333528952No Hit
TAGCTTTGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT155350.21451271369284292No Hit
TAGCTTAGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTT149000.20574441158824328No Hit
TAGCTTAGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGAT147420.20356269232442165No Hit
TAGCTTAGTGGGAGTTGCTCTAGCTCTTCTTTTCCCCTCTGATCACCTAC147040.2030379750331228No Hit
TAGCTTTGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG145410.20078721402044605No Hit
TAGCTTACCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTTC143020.19748701842517152No Hit
TAGCTTTCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCAC138000.19055522684011797No Hit
TAGCTTAATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC136920.18906392506484745No Hit
TAGCTTATTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTAC131700.18185596648437344No Hit
TAGCTTAGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTT131100.18102746549811205No Hit
TAGCTTAGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGA129630.1789976380817717No Hit
TAGCTTAGGGGTACAAAGGTTATGGGACTCATCAACCAAGTTGGATTCAG127170.1756007840381No Hit
TAGCTTAGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGT125940.17390235701626416No Hit
TAGCTTACCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCAT124780.1723005884428255No Hit
TAGCTTTCGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATG124110.17137542900816694No Hit
TAGCTTACCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC123500.17053311967213455No Hit
TAGCTTAGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC119020.16434697897471623No Hit
TAGCTTACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT114280.15780182118325128No Hit
TAGCTTTCCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTT111230.15359027450308926No Hit
TAGCTTTCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGC109090.15063528765209033No Hit
TAGCTTACTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGAAAGCTA108680.15006914531147839No Hit
TAGCTTAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAA108670.15005533696170736No Hit
TAGCTTAGTCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCA106960.14769410915086242No Hit
TAGCTTAGCCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCT102450.14146654340413103No Hit
TAGCTTACTGCAATTCACATTACGTATCGCATTTCGCTGCGTTCTTCATC101030.13950575773664578No Hit
TAGCTTAGCCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACA100790.1391743573421412No Hit
TAGCTTAGCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAAC98760.13637126233862354No Hit
TAGCTTAGGTCAGGCTCTTACCAGCTTAACTACAGTTGATCGGACGGGAA97700.1349075772628951No Hit
TAGCTTACCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAA97000.1339409927789235No Hit
TAGCTTAGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGG96520.13327819198991436No Hit
TAGCTTAGGCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGC96270.13293298324563882No Hit
TAGCTTTGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA96040.13261539120090526No Hit
TAGCTTTCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC92410.1276029602340239No Hit
TAGCTTAGCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGAAAGCT92140.12723013479020628No Hit
TAGCTTAGCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAA92100.1271749013911222No Hit
TAGCTTTACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCT91760.1267054174989074No Hit
TAGCTTACTGGGGTACAAAGGTTATGGGACTCATCAACCAAGTTGGATTC91450.1262773586560057No Hit
TAGCTTAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCA90540.1250207988268426No Hit
TAGCTTAGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTT90300.12468939843233803No Hit
TAGCTTACTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTAAAGCT88890.1227424211146238No Hit
TAGCTTAGCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGG88680.12245244576943233No Hit
TAGCTTAACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGG88100.12165156148271297No Hit
TAGCTTACTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA88070.12161013643339992No Hit
TAGCTTAGCCGACTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGT86700.11971839251476975No Hit
TAGCTTACACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGCG84330.11644581361903729No Hit
TAGCTTTGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT83670.11553446253414977No Hit
TAGCTTTCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCC83630.11547922913506568No Hit
TAGCTTTCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT83570.11539637903643955No Hit
TAGCTTAGTCAGGCTCTTACCAGCTTAACTACAGTTGATCGGACGGGAAA83230.11492689514422476No Hit
TAGCTTTATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC83100.11474738659720146No Hit
TAGCTTATTTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC81800.11295230112696847No Hit
TAGCTTAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACT81130.11202714169230993No Hit
TAGCTTAGGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA79550.10984542242848827No Hit
TAGCTTAGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTC79390.10962448883215191No Hit
TAGCTTAACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGCGT77220.10662807693183991No Hit
TAGCTTACCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACT77180.10657284353275583No Hit
TAGCTTAGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTT76930.10622763478848024No Hit
TAGCTTTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT76490.10562006739855523No Hit
TAGCTTACGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTTCA75140.10375594017946713No Hit
TAGCTTAGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAG75070.10365928173106996No Hit
TAGCTTACGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCT74510.10288601414389267No Hit
TAGCTTAGCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGCA74270.10255461374938811No Hit
TAGCTTTCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT73160.10102188692480456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAC1721000.0144.817322
AGCTTAG3260500.0144.795072
TAGCTTA5917400.0144.734021
GCTTAGG1123050.0144.712743
GCTTAGT1007750.0144.603353
GCTTAGC941500.0144.461533
GCTTACG547100.0144.28843
AGTGCGT457400.0144.260247
TAGGCGC148600.0144.245676
AGCTTTC704850.0144.154652
TTAGTGC495600.0143.982795
TAGTGCG468500.0143.956196
CTTAGGG469800.0143.665854
TTAGGCG261400.0143.618585
TAGGGCG212550.0143.57916
CTTAGTG619950.0143.457324
CTTAGCC342200.0143.1984
TACGCGC71100.0143.142236
AGGCGCA132650.0142.951497
AGGGCGC146100.0142.839957
Produced by FastQC (version 0.11.2)