FastQCFastQC Report
Thu 6 Oct 2016
HG5MKAFXX_n01_gln3_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5MKAFXX_n01_gln3_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10696219
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAAGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC5517425.158290046230355No Hit
CTCAGAAGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT2074931.9398723979006038No Hit
CTCAGATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC1431291.3381270521854498No Hit
CTCAGAAGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT1193201.1155343771476631No Hit
CTCAGAACGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT803050.750779317439181No Hit
CTCAGATCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT769500.7194130935426808No Hit
CTCAGAAGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTC741510.6932449681518301No Hit
CTCAGAAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT737800.6897764527820532No Hit
CTCAGAAGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG690690.6457328519545084No Hit
CTCAGAAGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA658790.6159092292332459No Hit
CTCAGAAGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA581960.5440801090553588No Hit
CTCAGATGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT555410.5192582537810791No Hit
CTCAGAAGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG436090.40770481606631276No Hit
CTCAGAATGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA432190.4040586678339327No Hit
CTCAGAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCG430860.4028152377957108No Hit
CTCAGATATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC368550.3445610079599155No Hit
CTCAGAAGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA316560.29595504729287986No Hit
CTCAGAACGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT309010.28889647827891335No Hit
CTCAGAAATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC297690.2783132993069794No Hit
CTCAGAAGTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAA297270.27792063718964616No Hit
CTCAGATGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG278140.2600358126549204No Hit
CTCAGAAGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGAT265360.24808766537035193No Hit
CTCAGAAGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTC254750.23816827235867177No Hit
CTCAGATCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT244020.22813669017061078No Hit
CTCAGAATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCA227270.21247695096744No Hit
CTCAGATGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTC207180.19369461302166682No Hit
CTCAGATGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT200790.18772053937938257No Hit
CTCAGAAGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGAC197660.18479427169544677No Hit
CTCAGAACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT195600.18286835750090757No Hit
CTCAGATATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC195120.18241960079538386No Hit
CTCAGATGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG193540.18094244330636836No Hit
CTCAGAAACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCA185160.1731078991557671No Hit
CTCAGAAATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC184410.17240671680338632No Hit
CTCAGATCGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT176240.16476850371145169No Hit
CTCAGAAGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAG171210.16006590740148458No Hit
CTCAGAAGGCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGC168940.15794366214827876No Hit
CTCAGAAGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGT161350.15084769674218526No Hit
CTCAGATGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA161310.1508103003500583No Hit
CTCAGAAGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGA159490.14910876450828092No Hit
CTCAGATCGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCA147590.13798333785050587No Hit
CTCAGAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCA135120.12632501260492143No Hit
CTCAGAAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCA132320.12370726515603318No Hit
CTCAGATCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT132120.1235202831953983No Hit
CTCAGAAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATC130890.12237034413749381No Hit
CTCAGAAGGTCAGGCTCTTACCAGCTTAACTACAGTTGATCGGACGGGAA130260.12178135096149395No Hit
CTCAGAAGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTT108430.10137226995819738No Hit
CTCAGATTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA107740.10072718219400706No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAA7249400.0144.86681
AGAAGGG587150.0144.271584
GAAGTGC609750.0144.143575
TCAGAAG3951350.0144.048322
CTCAGAT3421450.0143.866441
TCAGATG991500.0143.635062
AAGTGCG585750.0143.428286
GAAGGGC362300.0143.366935
CAGAAGT1176800.0143.041053
AGTGCGT573050.0143.024127
CAGATCG598950.0142.97853
CAGAAGC1263900.0142.865463
GAACGCG91250.0142.842675
AGGGCGC218100.0142.678867
TCAGAAT719700.0142.409062
GAAGGCG266800.0142.406375
TCAGATA401200.0142.403782
GAAGCCC233200.0142.252065
AGAAGGC480600.0142.166964
AGAAGCC501250.0142.066254
Produced by FastQC (version 0.11.2)