FastQCFastQC Report
Thu 6 Oct 2016
HG5MKAFXX_n01_gln3_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5MKAFXX_n01_gln3_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAGTCCAAATGTTTTTGAGATGGCAGCAACGGAAACCATAACGAGCA1137.880055788005579No Hit
GATGAGTCGAAAAATTATCTTGATAAAGCAGGAATTACTACTGCTTGTTT926.4156206415620645No Hit
GATGAGTATAATTACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAG906.2761506276150625No Hit
GATGAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG815.648535564853557No Hit
GAGCAGTAGACTCCTTCTGTTGATAAGCAAGCATCTCATTTTGTGCATAT805.578800557880056No Hit
GGTCAGTATGCAAATTAGCATAAGCAGCTTGCAGACCCATAATGTCAATA805.578800557880056No Hit
GCTCAGTCTCAGGAGGAAGCGGAGCAGTCCAAATGTTTTTGAGATGGCAG704.881450488145049No Hit
TATCAGTATTTTTGTGTGCCTGAGTATGGTACAGCTAATGGCCGTCTTCA694.811715481171548No Hit
GGTCAGTAGCAATCCAAACTTTGTTACTCGTCAGAAAATCGAAATCATCT644.463040446304045No Hit
GGTCAGTATTTTACCAATGACCAAATCAAAGAAATGACTCGCAAGGTTAG412.8591352859135286No Hit
GGTCAGTTCCATCAACATCATAGCCAGATGCCCAGAGATTAGAGCGCATG412.8591352859135286No Hit
GATGAGTTTGTATCTGTTACTGAGAAGTTAATGGATGAATTGGCACAATG392.7196652719665275No Hit
GAGCAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG392.7196652719665275No Hit
GATTAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG392.7196652719665275No Hit
GTTCAGTAACTTGACTCATGATTTCTTACCTATTAGTGGTTGAACAGCAT372.580195258019526No Hit
GATAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAA292.0223152022315203No Hit
GATGAGTTTAATTACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAG292.0223152022315203No Hit
GGTCAGTTTGCAAATTAGCATAAGCAGCTTGCAGACCCATAATGTCAATA271.882845188284519No Hit
GGTCAGTTGCAATCCAAACTTTGTTACTCGTCAGAAAATCGAAATCATCT261.813110181311018No Hit
GTTCAGTTGTTGCAGTGGAATAGTCAGGTTAAATTTAATGTGACCGTTTA261.813110181311018No Hit
TATCAGTTTTTTTGTGTGCCTGAGTATGGTACAGCTAATGGCCGTCTTCA241.6736401673640167No Hit
GATGAGTGTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGCGTAGAGG201.394700139470014No Hit
CATCAGTGACGACATTAGAAATATCCTTTGCAGTAGCGCCAATATGAGAA191.3249651324965133No Hit
GAGCAGTTGACTCCTTCTGTTGATAAGCAAGCATCTCATTTTGTGCATAT181.2552301255230125No Hit
GACCAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG171.1854951185495117No Hit
GGTCAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161.1157601115760112No Hit
GAACAGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCC151.0460251046025104No Hit
GTTCAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140.9762900976290098No Hit
AATCAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110.7670850767085077No Hit
GGTCAGTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCT110.7670850767085077No Hit
GTTCAGTTACTTGACTCATGATTTCTTACCTATTAGTGGTTGAACAGCAT100.697350069735007No Hit
GACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGAATAGTCAGGTTAA100.697350069735007No Hit
GATCGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90.6276150627615062No Hit
GATAAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70.4881450488145049No Hit
GATGAGTATGTATCTGTTACTGAGAAGTTAATGGATGAATTGGCACAATG50.3486750348675035No Hit
GGTCAGTTTTTTACCAATGACCAAATCAAAGAAATGACTCGCAAGGTTAG50.3486750348675035No Hit
GATCGGTATAAGTCAAAGCACCTTTAGCGTTAAGGTACTGAATCTCTTTA40.2789400278940028No Hit
GATAAGTAGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAA30.20920502092050208No Hit
GATCTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.20920502092050208No Hit
CATCAGTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1394700139470014No Hit
GAACAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1394700139470014No Hit
GATCAGCGTTAATCGTGCCAAGAAAAGCGGCATGGTCAATATAACCAGTA20.1394700139470014No Hit
GTTCAGTCGGCGACTTCACGCCAGAATACGAAAGACCAGGTATATGCACA20.1394700139470014No Hit
GACCAGTAGTGTTAACAGTCGGGAGAGGAGTGGCATTAACACCATCCTTC20.1394700139470014No Hit
GATCTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAATGA20.1394700139470014No Hit
GGTCAGTACCATCAACATCATAGCCAGATGCCCAGAGATTAGAGCGCATG20.1394700139470014No Hit
GGTCAGTAGGAATCCAAACTTTGTTACTCGTCAGAAAATCGAAATCATCT20.1394700139470014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGACT100.006357185145.06
GTTTGTA100.006357185145.06
TTGCAAA100.006357185145.0145
TTGTATC100.006357185145.08
TTTCTTA252.104025E-8145.0145
ACTGACC100.006357185145.0145
AGTAGCA252.104025E-8145.05
CAGTATT159.859691E-5145.04
AGTAGAC100.006357185145.05
TGTCCAG159.859691E-5145.0145
ACGCAGT100.006357185145.0145
CAGTAGA100.006357185145.04
GTATTTT159.859691E-5145.06
AGTGGGG201.4667385E-6145.05
TTTTTGT159.859691E-5145.09
GGTCAGT353.6379788E-12145.01
CATCAGT100.006357185145.01
GAGTTTG100.006357185145.04
GCAATCC252.104025E-8145.09
AGTATTT159.859691E-5145.05
Produced by FastQC (version 0.11.2)