FastQCFastQC Report
Thu 6 Oct 2016
HG5MKAFXX_n01_gln3_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5MKAFXX_n01_gln3_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6738679
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGAGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC1837342.726558128084154No Hit
ACAGTGAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT1711432.539711418217131No Hit
ACAGTGAGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA1390142.0629265765589966No Hit
ACAGTGACCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG1176991.7466182912110815No Hit
ACAGTGAGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG943501.4001260484436193No Hit
ACAGTGAGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA924261.3715744584361416No Hit
ACAGTGACGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC914811.3575509383960862No Hit
ACAGTGAGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC913771.3560076092064928No Hit
ACAGTGAGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG879151.3046325548375282No Hit
ACAGTGAGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT727741.0799446004179751No Hit
ACAGTGACCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG663250.9842433509594388No Hit
ACAGTGTCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG589880.8753644445743743No Hit
ACAGTGAGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGAT578870.859025930749929No Hit
ACAGTGAGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA574910.8531494080664771No Hit
ACAGTGAGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGAT526790.7817407536402907No Hit
ACAGTGTCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC507260.7527588122241763No Hit
ACAGTGAGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT492540.7309147683099313No Hit
ACAGTGAGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG391430.5808705237332125No Hit
ACAGTGACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCAC379610.5633299939053337No Hit
ACAGTGACGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC360870.5355203890851604No Hit
ACAGTGTCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG337570.5009438793567701No Hit
ACAGTGACCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGC312220.46332523036043116No Hit
ACAGTGAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCA310590.46090635864981844No Hit
ACAGTGAACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCT308220.45738934886199506No Hit
ACAGTGACTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT300560.44602213579248995No Hit
ACAGTGACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGA297620.4416592628911394No Hit
ACAGTGACACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCC270870.40196305537034777No Hit
ACAGTGAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCG262390.3893789865936632No Hit
ACAGTGACGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT250160.3712300289121948No Hit
ACAGTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT250010.37100743335600345No Hit
ACAGTGAGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGA241810.3588388762842094No Hit
ACAGTGACCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC234350.347768457289626No Hit
ACAGTGTCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCAC227010.3368761147399958No Hit
ACAGTGACCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCAT221870.32924850701450536No Hit
ACAGTGATGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA211550.3139339327485402No Hit
ACAGTGTCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC208430.30930394517976No Hit
ACAGTGACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTA202500.30050400085832846No Hit
ACAGTGAGCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAAC198810.29502815017602113No Hit
ACAGTGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATG194330.2883799628977727No Hit
ACAGTGTGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC188500.2797284156138021No Hit
ACAGTGTGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT179250.2660016896486685No Hit
ACAGTGACAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATT178540.2649480706826961No Hit
ACAGTGAGTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAA175680.2607039154113143No Hit
ACAGTGAGGAACTATCAAATAAACGATAACTGATTTAATGAGCCATTCGC173030.2567713939186004No Hit
ACAGTGAGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGC172830.2564745998436786No Hit
ACAGTGTCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGA164400.24396472958572446No Hit
ACAGTGAGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTT164130.24356405758458No Hit
ACAGTGAGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAG161370.23946829935065908No Hit
ACAGTGAGGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCG159340.23645583949020274No Hit
ACAGTGTCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT154950.2299412095456691No Hit
ACAGTGTCGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT151970.2255189778293342No Hit
ACAGTGTCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGC151220.22440600004837744No Hit
ACAGTGAGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTC151060.22416856478844No Hit
ACAGTGTACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCT146960.21808428625254297No Hit
ACAGTGTGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA146940.21805460684505082No Hit
ACAGTGAAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCAT132990.19735322011925482No Hit
ACAGTGTCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCC132020.19591376885588407No Hit
ACAGTGAGCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAA131210.19471175285245076No Hit
ACAGTGTCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC130160.19315358395911128No Hit
ACAGTGACCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAA130050.19299034721790428No Hit
ACAGTGAGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGG129930.19281227077295118No Hit
ACAGTGAGCCGACTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGT128160.1901856432098932No Hit
ACAGTGACCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACT127660.1894436580225887No Hit
ACAGTGATGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC127350.1889836272064599No Hit
ACAGTGAGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGT126740.1880784052779484No Hit
ACAGTGTCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCAT125610.1864015187546402No Hit
ACAGTGAGCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGG125460.18617892319844884No Hit
ACAGTGACCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGG124920.18537757919615996No Hit
ACAGTGACGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT123510.18328518096796123No Hit
ACAGTGAGAATTACCGCGGTTATACCATGTAGTAAAGGAACTATCAAATA122490.18177153118586004No Hit
ACAGTGACCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTT117190.17390648820043217No Hit
ACAGTGAGTCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCA117020.17365421323674862No Hit
ACAGTGTCCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTA116990.17360969412551036No Hit
ACAGTGACCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACC115270.17105726508118282No Hit
ACAGTGAGGCGGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA114760.17030044019013224No Hit
ACAGTGAGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC111650.16568529232509815No Hit
ACAGTGAGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGC109370.16230183987098956No Hit
ACAGTGTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT108920.1616340532024155No Hit
ACAGTGAACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGG108890.1615895340911772No Hit
ACAGTGTCGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATG108490.16099594594133362No Hit
ACAGTGAGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCT108430.16090690771885707No Hit
ACAGTGAGAGCTGGAATTACCGCGGTTATACCATGTAGTAAAGGAACTAT108170.16052107542145871No Hit
ACAGTGAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACT107840.16003136519783775No Hit
ACAGTGATGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG106550.1581170434145921No Hit
ACAGTGTCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATT106530.1580873640070999No Hit
ACAGTGTGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG105460.15649951570626824No Hit
ACAGTGAATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC103500.15359093377203453No Hit
ACAGTGTGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC102490.15209212369367944No Hit
ACAGTGAAGCTGGAATTACCGCGGTTATACCATGTAGTAAAGGAACTATC100420.14902030501823874No Hit
ACAGTGAGCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCA100190.14867899183207867No Hit
ACAGTGTGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA96810.14366317196590014No Hit
ACAGTGTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG95770.14211984277630674No Hit
ACAGTGACCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAA94100.13964161225070967No Hit
ACAGTGAGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTT92380.13708918320638214No Hit
ACAGTGACCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTG90990.1350264643856756No Hit
ACAGTGAGGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC88710.13164301193156702No Hit
ACAGTGAGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTA88410.1311978208191843No Hit
ACAGTGAGCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGCA87990.1305745532618485No Hit
ACAGTGAGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATAC87880.1304113165206415No Hit
ACAGTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCCGGTGAGTTG87720.13017388126070406No Hit
ACAGTGAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAG83730.12425283946601404No Hit
ACAGTGATCGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCT83080.12328825872251817No Hit
ACAGTGTGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT82860.12296178524010418No Hit
ACAGTGAGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT81720.12127005901304988No Hit
ACAGTGACAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAAA81170.12045387530701492No Hit
ACAGTGAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAA80790.11988996656466348No Hit
ACAGTGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCA80060.1188066681911989No Hit
ACAGTGAGCGCCCGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT79820.11845051530129273No Hit
ACAGTGACGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTAT79800.11842083589380056No Hit
ACAGTGACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTT79670.11822791974510137No Hit
ACAGTGAGCTGGAATTACCGCGGTTATACCATGTAGTAAAGGAACTATCA77940.11566065099702776No Hit
ACAGTGAAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC77700.11530449810712158No Hit
ACAGTGTCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGG76880.11408764239994218No Hit
ACAGTGTCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAA76690.11380568802876648No Hit
ACAGTGACCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCA76280.11319726017517677No Hit
ACAGTGAGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTT75140.11150553394812249No Hit
ACAGTGACCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAA74970.11125325898443893No Hit
ACAGTGTCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACC74920.1111790604657085No Hit
ACAGTGATTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTAC74000.10981380772106819No Hit
ACAGTGAGGCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGC72730.10792916534531471No Hit
ACAGTGAGTTCTGCTTACCAAAAATGGCCCACTAAAAGCTCTTCATTCAA72100.10699426400931102No Hit
ACAGTGATGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC71650.10632647734073696No Hit
ACAGTGAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATC71330.10585160682086206No Hit
ACAGTGAGCGGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGAT70560.10470894963241313No Hit
ACAGTGAGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCAT69330.10288366607164401No Hit
ACAGTGACCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACG68520.10168165006821069No Hit
ACAGTGAGCGCGTCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG68230.10125129865957408No Hit
ACAGTGACGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG68040.10096934428839838No Hit
ACAGTGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC67980.10088030606592183No Hit
ACAGTGAGGGTGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG67790.1005983516947461No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGAG2889700.0144.865842
AGTGAGG909250.0144.630923
AGTGAGC1194200.0144.627723
TGACGCG134100.0144.541285
AGTGACC767700.0144.539373
TGAGGGC230300.0144.470115
ACAGTGA5260000.0144.431371
TGAGGCG195900.0144.418675
GTGACCC559150.0144.416264
GTGAGCC398850.0144.355794
TGAGTGC231450.0144.347355
GTGAGGC452100.0144.268234
GTGAGTG301000.0144.179024
GTGACGC257850.0144.158484
GTGAGCG580850.0144.150074
TGACCCC267900.0144.134935
CAGTGTC900200.0144.104062
CAGTGAC1616500.0144.099292
AGTGAGT596350.0144.08643
ACAGTGT1462950.0144.084371
Produced by FastQC (version 0.11.2)