FastQCFastQC Report
Thu 6 Oct 2016
HG5MKAFXX_n01_gln3_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5MKAFXX_n01_gln3_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10728518
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACCAAGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC4958384.621682137271895No Hit
TGACCAAGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT1880921.7531964806322737No Hit
TGACCAAGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG1664961.5519012038755027No Hit
TGACCAAGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA1659621.5469238155726635No Hit
TGACCAAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT1412171.3162768613521458No Hit
TGACCAAGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT1078621.0053765114622542No Hit
TGACCAACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATG1036090.9657345031252219No Hit
TGACCATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC999650.9317689544818772No Hit
TGACCAAGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGT863150.804537961347504No Hit
TGACCAAGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG792320.7385176591957994No Hit
TGACCAATGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA694660.6474892431554852No Hit
TGACCAAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCG692530.6455038804054763No Hit
TGACCAAGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGC653290.6089284652362983No Hit
TGACCAACGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT584950.5452290801022098No Hit
TGACCAAGGAACTATCAAATAAACGATAACTGATTTAATGAGCCATTCGC560700.5226257717981179No Hit
TGACCAAGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA554390.5167442511631151No Hit
TGACCAAGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG445410.4151645175969319No Hit
TGACCAAGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTT415450.38723894577051554No Hit
TGACCATGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT396650.36971555623992053No Hit
TGACCAAGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGAT368900.343849914778537No Hit
TGACCAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAA341470.3182825437772486No Hit
TGACCAAATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC334660.3119349755483469No Hit
TGACCATGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA334370.3116646679438856No Hit
TGACCATGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG322720.3008057590060435No Hit
TGACCAAGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTC301250.28079367532402894No Hit
TGACCAAGAATTACCGCGGTTATACCATGTAGTAAAGGAACTATCAAATA301150.28070046580524916No Hit
TGACCATGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA297780.2775593050223712No Hit
TGACCATATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC267870.24968033795534483No Hit
TGACCAACTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT261250.2435098678121247No Hit
TGACCAAGTTCTGCTTACCAAAAATGGCCCACTAAAAGCTCTTCATTCAA252420.2352794673038718No Hit
TGACCAACAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATT250340.23334070931325276No Hit
TGACCATCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT250250.233256820746351No Hit
TGACCAACGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT248100.23125281609258616No Hit
TGACCAAAGCTGGAATTACCGCGGTTATACCATGTAGTAAAGGAACTATC237640.2215031004282232No Hit
TGACCAACGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTAT231510.21578935692702383No Hit
TGACCAAGTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAA227040.21162289143756854No Hit
TGACCATATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAA210570.19627128369454197No Hit
TGACCAAGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC205560.19160148680367597No Hit
TGACCAAACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCT199870.18629786518510758No Hit
TGACCATCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATT190430.17749888661229818No Hit
TGACCAAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAG178170.16607139960989953No Hit
TGACCATCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT168140.15672248487628954No Hit
TGACCATGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCG158220.14747610061333727No Hit
TGACCAACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT157380.14669314065558728No Hit
TGACCAAGAGCTGGAATTACCGCGGTTATACCATGTAGTAAAGGAACTAT153480.1430579694231766No Hit
TGACCATCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTAT148630.13853730776235823No Hit
TGACCAAGCGGTTATCAGTACGACCTGGCATGAAAACTATTCCTTCCTGT146140.13621639074474218No Hit
TGACCAAGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAG142700.1330099832987184No Hit
TGACCAAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCA142630.1329447366355726No Hit
TGACCAAATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC142590.13290745282806068No Hit
TGACCAAGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGA141830.1321990604853345No Hit
TGACCAAGGGGGTATGAACAACAATTGTTGTAGTCGCAACTACGGTAATT139530.1300552415534No Hit
TGACCAATGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC130920.12202990198646262No Hit
TGACCAAGCTGGAATTACCGCGGTTATACCATGTAGTAAAGGAACTATCA124740.11626955372587341No Hit
TGACCATGGAACTATCAAATAAACGATAACTGATTTAATGAGCCATTCGC118060.1100431578713854No Hit
TGACCAAGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTA115770.10790865989132889No Hit
TGACCATGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGT112270.10464632673403726No Hit
TGACCATTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA110960.10342528203802241No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCGT518300.0144.090737
AAGTGCG537100.0143.529776
AGGGCGC194700.0143.519877
CCAAGCG718950.0143.05824
TGACCAA8309600.0142.87861
AAGGCGC201600.0142.816486
AGGCGCA177500.0142.766627
GACCAAC2066750.0142.52292
CAACGCG166950.0142.02145
GACCAAT796750.0141.599212
CAAGCGG147400.0141.482255
ACCAACG789200.0141.244783
TGACCAT2549950.0141.061871
CAAGCGT155350.0140.914385
GACCAAG4936150.0140.278242
CAAGGCG347650.0140.136525
GACCATG957500.0139.902282
GACCAAA629900.0138.771842
CAAGTTC266900.0138.672995
ACCATGC258850.0138.645053
Produced by FastQC (version 0.11.2)