FastQCFastQC Report
Thu 6 Oct 2016
HG5MKAFXX_n01_gln3_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5MKAFXX_n01_gln3_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10100332
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATGTAGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC4361124.317798662459809No Hit
CGATGTAGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA2277182.254559553091918No Hit
CGATGTAGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT1571191.5555825293663614No Hit
CGATGTAGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA975210.9655227174710692No Hit
CGATGTACGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC826220.818012714829572No Hit
CGATGTAGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACAT794410.7865187005734069No Hit
CGATGTAGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA716850.7097291455369982No Hit
CGATGTAGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGAT629910.6236527670575581No Hit
CGATGTAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCG605570.5995545492960034No Hit
CGATGTAATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC536250.5309231419323642No Hit
CGATGTAGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG525630.5204086360725568No Hit
CGATGTAGGAACTATCAAATAAACGATAACTGATTTAATGAGCCATTCGC512090.507003136134535No Hit
CGATGTACGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT484580.4797664076784803No Hit
CGATGTTGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC478150.4734002803076176No Hit
CGATGTAGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTC403580.39957102400198335No Hit
CGATGTAGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTT361720.3581268417711418No Hit
CGATGTTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC307540.3044850406897516No Hit
CGATGTAGTTCTGCTTACCAAAAATGGCCCACTAAAAGCTCTTCATTCAA302660.2996535163398589No Hit
CGATGTAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAA300790.2978020920500435No Hit
CGATGTTGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA273110.2703970522949147No Hit
CGATGTAGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTC267280.26462496480313713No Hit
CGATGTAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAG266440.26379330897241793No Hit
CGATGTACTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT251900.24939774256925418No Hit
CGATGTAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCA234020.2316953541725163No Hit
CGATGTAAGCTGGAATTACCGCGGTTATACCATGTAGTAAAGGAACTATC229490.2272103530854233No Hit
CGATGTAGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGA225320.22308177592578146No Hit
CGATGTTGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG220370.21818094692332887No Hit
CGATGTAGAATTACCGCGGTTATACCATGTAGTAAAGGAACTATCAAATA211040.20894362680355458No Hit
CGATGTACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT195540.19359759659385453No Hit
CGATGTTGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT193780.19185507961520473No Hit
CGATGTACGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATT189380.1874987871685802No Hit
CGATGTAGAGCTGGAATTACCGCGGTTATACCATGTAGTAAAGGAACTAT183630.18180590499401406No Hit
CGATGTAGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC182620.18080593786422072No Hit
CGATGTTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT180270.17847928167113714No Hit
CGATGTAGGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCG153170.15164848046579063No Hit
CGATGTAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACT151580.15007427478621493No Hit
CGATGTTATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAA149750.14826245315500522No Hit
CGATGTAATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC136480.13512427116257167No Hit
CGATGTAGGGGGTATGAACAACAATTGTTGTAGTCGCAACTACGGTAATT132560.1312432106192153No Hit
CGATGTAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCG125040.12379791080134792No Hit
CGATGTAGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAG124990.12374840747809081No Hit
CGATGTTCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT123790.12256032771992051No Hit
CGATGTACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAG123630.12240191708549777No Hit
CGATGTAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATC120790.11959012832449468No Hit
CGATGTAGCGGTTATCAGTACGACCTGGCATGAAAACTATTCCTTCCTGT118360.11718426681419977No Hit
CGATGTAGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGC114810.11366953086294589No Hit
CGATGTAGGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA112730.11161019261545066No Hit
CGATGTTCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATT112170.11105575539497117No Hit
CGATGTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG109910.10881820518375039No Hit
CGATGTAGGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC109210.10812515865815105No Hit
CGATGTTAGCTGGAATTACCGCGGTTATACCATGTAGTAAAGGAACTATC108650.10757072143767156No Hit
CGATGTATTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTAC106520.10546187986691923No Hit
CGATGTTCGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT105060.10401638282781199No Hit
CGATGTTGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTA102410.10139270669518585No Hit
CGATGTATGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCA102240.10122439539611172No Hit
CGATGTACCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGT101830.10081846814540354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTA8051550.0144.868211
GATGTAG4786500.0144.700532
ATGTAGG1693950.0144.589513
ATGTAGC1492750.0144.561873
GATGTAC1972550.0144.541022
TGTAGGG549000.0144.274864
GTAGTGC508950.0144.262515
CGATGTT2017400.0144.206421
GTACGCG143950.0144.168965
TGTAGGC880650.0144.135074
TAGTGCG468100.0144.107456
TAGGCGC247000.0144.035546
GATGTTC986650.0144.026922
AGTGCGT451750.0143.979087
GTAGGGC312500.0143.838045
GTAGGCG408050.0143.784445
TGTAGTG653250.0143.698564
TGTAGCC533200.0143.53364
GTACCCC194750.0143.523035
GTAGGGG141150.0143.382645
Produced by FastQC (version 0.11.2)