FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s96.352000000d870f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s96.352000000d870f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTACGTTCATGCTGCCAATGAAGTGTCCAAAACCTTGGCCGCTGTACC29.523809523809524No Hit
GTCTCCGAGTGCCGATCCCAAGCTTAGCATCGCTGAGAGTTTCCGTGCGG29.523809523809524No Hit
CTACCACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTG14.761904761904762No Hit
TAAATAGCTTGCAAAATACGTGGCCTTATGGTTACAGTATGCCCATCGCA14.761904761904762No Hit
GTACCCGGAGTGAGTGTAGATCTCGGTGGCCGCCGGATGATTAAAAAAGA14.761904761904762Illumina Single End PCR Primer 1 (95% over 24bp)
GGTGGAGTGTCTCCGAGTGCCGATCCCAAGCTTAGCATCGCTGAGAGTTT14.761904761904762No Hit
TTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTGCAATTAAAA14.761904761904762No Hit
ACCCTGAAACAAATGCTTAGGGATTTTATTGGTATCAGGGTTAATCGTGC14.761904761904762No Hit
GCCGTCATTGCTTATTATGTTCATCCCGTCAACATTCAAACGGCCTGTCT14.761904761904762No Hit
TTTTAAAGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGC14.761904761904762No Hit
AAGAAGCTGCGTGCCCAGAAGCAGCTGCGTCTGTCCAGCGTCACCGGTGG14.761904761904762No Hit
GTCCAGCGCCACCGGTGGAGTGTCTCCGAGTGCCGATCCCAAGCTTAGCA14.761904761904762No Hit
TGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAAAGCCGCCTCCG14.761904761904762No Hit
TCGTGATATTGGTCGTATGGTTCTTGCTGCCGAGGGTCAAAAGGCTAATG14.761904761904762No Hit
GCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCG14.761904761904762No Hit
TAGTTGAGACGATGGCCAAGGTGACTCATCAGCTGGGTGCCGAGATCCAC14.761904761904762No Hit
CCGTGGACAGATTTGTCATTGTGAGCATTTTCATCCCGAAGTTGCGGCTC14.761904761904762No Hit
GGACGAGCTCAAGGACATCATGGTGGAGTGCCTGACCCAGGAGGGCGAGC14.761904761904762No Hit
AAAGATTGGTGTTTTCCATAATAGACGCAACGCGAGCAGTAGACTCCTTC14.761904761904762No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)