FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s94.352000000d86ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s94.352000000d86ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12459737
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT707630.5679333359925656No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA608990.48876633591864743No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT529090.42463978172251954No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA436550.3503685511178928No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA139270.11177603508003418No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC483150.041.659366
GGAGACG499550.039.141487
AGGGAGA223300.038.5816155
GAGACGT475750.038.1845788
AGACGTG453500.036.622989
TGGGAGA195000.033.6593175
CAGGGAG146950.032.6101954
TAGGGAG56050.031.5209434
CCGATCT740450.028.9917564
CGATCTG342400.028.8094255
TTCCGAT765050.028.2892272
TCCGATC792050.027.4687923
AGACGTT57750.025.4944159
CTGGGAG158050.023.5888634
GATCTGG147150.023.1738266
TCCGAAC102800.022.8226323
CTTCCGA1086200.022.2906061
CGATCTA173450.021.8778345
ACAGGGA71700.020.9314733
CCAGGGA97950.020.6555613
Produced by FastQC (version 0.11.2)