FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s9.352000000d7bff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s9.352000000d7bff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGT90.4524886877828055No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA70.35193564605329314No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.35193564605329314No Hit
GGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATA50.2513826043237808No Hit
GTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACT30.1508295625942685No Hit
ACTCTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTT30.1508295625942685No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA30.1508295625942685No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA30.1508295625942685No Hit
GTAGTATCTAGGACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGA30.1508295625942685No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT30.1508295625942685No Hit
GAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAG30.1508295625942685No Hit
CTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAA30.1508295625942685No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG20.10055304172951231No Hit
TCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAA20.10055304172951231No Hit
TCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTA20.10055304172951231No Hit
ATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAA20.10055304172951231No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT20.10055304172951231No Hit
GAGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTA20.10055304172951231No Hit
ATCTAGGACTACCCTGATTCCCCGTTACCCGTTGCAACCATGGTAGTCCT20.10055304172951231No Hit
GATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGT20.10055304172951231No Hit
GCACAGGGAGACGTGTGCTCTTCCGATCTGCACAGGGAGACGTGTGCTCT20.10055304172951231Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAA20.10055304172951231No Hit
TCCCCGAGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATG20.10055304172951231No Hit
ATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTT20.10055304172951231No Hit
CTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTC20.10055304172951231No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA20.10055304172951231No Hit
AGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAA20.10055304172951231No Hit
GCCATGGGAGACGTGTGCTCTTCCGATCTGCCATGGGAGACGTGTGCTCT20.10055304172951231Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAACTTTAAT20.10055304172951231No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA20.10055304172951231No Hit
CATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAA20.10055304172951231No Hit
TTTCAAATGTCTGCCCTATCAACTTTTGATGGTAGTATCTAGGACTACCA20.10055304172951231No Hit
GTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGTAAGTT20.10055304172951231No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers