FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s85.352000000d8595.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s85.352000000d8595.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTT17.6923076923076925No Hit
CTTCTGCACCTCAGCATTAGCAGCTGGGGCTTCAGGGGCCTGTTCAATGG17.6923076923076925No Hit
CCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGT17.6923076923076925No Hit
TAATCCTGTCCAAGGGGGCGAGTGGCTCCCCCAGCTGGCGGCCCAGGCGA17.6923076923076925No Hit
CGTCAGGATCGGTCGAATGTCCACCTCTTTCTATTCCCCTACCTACCGTT17.6923076923076925No Hit
GAAGGTGGGGAAAGGACGAATGCGCAACCACCCTGTGCGGTTTTTGGCGG17.6923076923076925No Hit
AGATATAACTACATGGAAAGATTTAAACCGGATTAATATTACATCCGTGT17.6923076923076925No Hit
AGTCAACTTAGAGTGCCCAACTGAATGATGGCAACTAAGCTTAAGGGTTG17.6923076923076925No Hit
CAAAGGCAAAATCAACCGAAACCACCCCCCCACCTCAGCACCCGTCCCCC17.6923076923076925No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT17.6923076923076925No Hit
TGTCTCTTATACACATCTTACGCTTCCGACGAAAACCCCTTTTTTAATCC17.6923076923076925No Hit
CTCTAAGTTGACTGCCGGTGAAAAACCGGAGGAAGGTGGGGATGACGTCA17.6923076923076925No Hit
CCCCTATTTCCTCCCACCAAGCCACGCAATCTTACGCTGTCTCTTATACA17.6923076923076925No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)