FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s70.352000000d8360.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s70.352000000d8360.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12689230
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT374480.2951164097427504No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA293860.23158221578456692No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT266680.21016247636775437No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT241350.190200666234279No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA201730.1589773374743779No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT714000.047.7526664
TTCCGAT728400.047.2259182
CGATCTG321000.046.8274545
TCCGATC745850.046.057343
CGATCTA164650.039.863755
GAGACGT234500.038.2580388
GGAGACG252400.037.802827
GGGAGAC262550.037.3362966
CTTCCGA955300.037.1673281
AGACGTG229900.036.1313679
CGATCTT133100.035.718065
CGATCTC223750.035.2776645
GATCTGG148200.033.2646336
AGGGAGA134150.033.0664865
GATCTCA70900.029.340096
GATCTGC152550.028.5489856
GATCTGA120900.028.519636
TGGGAGA116950.027.1680055
CAGGGAG98300.026.4763984
GATCTAG75800.025.8143736
Produced by FastQC (version 0.11.2)