FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s7.352000000d7bbb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s7.352000000d7bbb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGTGGTTGATGCGGTACAAGCTTTGACGGTGTAGATCTCGGTGTTGC15.263157894736842No Hit
TTCTTGGTCCGCTTGTCCTGCTCCTCGCTGCTCCCGCTTCCACTTCCGCC15.263157894736842No Hit
ATTCCAGCTCCAATAGCGTATATTAAAGTTGTTTACGGTTAAAACGTTCG15.263157894736842No Hit
GTACTTGTGTGTGTGTGTGTGTGTGAGTTCGGTACCCGAAAACGGAAGAA15.263157894736842No Hit
ATGATGGCCTAACTGCATCCTCAGATCGACTATTGTTGCTCCAAATGCTG15.263157894736842No Hit
CTGTGTGCCTGACCCTTACCGCCAAGCGTATGGCCTCAAAGACCTGTCTG15.263157894736842No Hit
GCACGTCTGCACCAAGATCGTGAATCAAAATGGCAAGAAGAAAACGCTGA15.263157894736842No Hit
AGTTGTTGGGGATTTACTTCCTTGGTAACGTAGCTAACGCGTGAAGTTGA15.263157894736842No Hit
AGAGCACACGTCTCCCTTAAGAGATCGGAAGAGCACACGACTCCCTTAAG15.263157894736842Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGAAGGAAATATGAAACAAAAAGAGAAACATTTACTAATAGGTACTGTTT15.263157894736842No Hit
ACCTTCTGGTTGAGGTCCACGATCATCTGGGAGTTCTCGCCCTTGCGCTT15.263157894736842No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG15.263157894736842No Hit
TATAGCCACTGTTTGTTCGCAGTGCACACAAAATGACTTGAGATATTTGC15.263157894736842No Hit
GTCGATATCTAAATGTGTGTGTATATCGATGTGATGTCATCGATTGTCGA15.263157894736842No Hit
GTATATTATTATCCGTACAAATTTGTTTCTCAGTTCTTTTTGAACACGGG15.263157894736842No Hit
CTTCCGATCTGAGCTGGGAGACGTGTGCTCTTCCGATCCTGTCTCTTATA15.263157894736842Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CATCGATGGCATTCACAAAGTTACTCAACGCCCTCGCTGCCGTTCGAGCT15.263157894736842No Hit
GTGCGACACCTGCTCTGCAATATAAAAGGTTCGGCTGCCGCTGGGATGCT15.263157894736842No Hit
TTATTATTATTTCTCTGGTGCCTTTCTGCTTTTTTCCACTGCAGTCTCGG15.263157894736842No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)