FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s69.352000000d8347.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s69.352000000d8347.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8399104
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT477160.5681082172574599No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA394270.46941911899174005No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT342430.4076982497180652No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA263310.3134977254716694No Hit
TCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTT143500.17085155749946662No Hit
CTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTC122500.14584889054832514No Hit
CTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTC117000.1393005730135024No Hit
GAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAG105910.1260967836569234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC162450.037.3054056
GAGACGT151450.037.19288
AGACGTG148900.035.0228169
CCGATCT353600.034.935664
GGAGACG173550.034.1259887
TCCGATC373350.033.5837063
TTCCGAT379100.033.224662
CGATCTG190550.031.7043615
CTTCCGA442000.029.5280861
AGGGAGA99500.028.210145
TAGGGAG33050.025.148234
TGGGAGA83050.023.7328175
CGATCTA87150.022.6162995
CAGGGAG66900.022.3627554
GATCTGG100000.021.2774126
CGATCTC131250.021.024115
CGATCTT94950.018.7575935
GATCTAG38850.018.337546
GTGCTCT277500.017.7654081
GTATATA60650.017.4628411
Produced by FastQC (version 0.11.2)