FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s66.352000000d82db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s66.352000000d82db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10739604
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA145020.13503291182803387No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT137810.12831944269081058No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC643450.044.5442476
GAGACGT632400.041.7112588
GGAGACG678700.041.3010037
AGGGAGA303400.040.7462585
CAGGGAG177400.037.8283774
AGACGTG646650.037.288699
TGGGAGA259450.036.976595
TAGGGAG80300.033.8897364
AGACGTT66300.031.6627049
CTAGGGA39100.028.7872853
CCAGGGA104050.026.3824353
TCAGGGA53100.026.206093
ACAGGGA72650.025.6912983
TTGGGAG102400.024.488554
CGATCTG403950.023.6906285
CCGATCT893350.023.338454
AAGGGAG103200.023.1942314
TTCCGAT911900.023.1761842
CTGGGAG220950.023.1070024
CCTGGGA88650.022.9830533
Produced by FastQC (version 0.11.2)