FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s64.352000000d8298.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s64.352000000d8298.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACGCAAGCCTCAACGCAGCGACGAGCACGAGAGCGGTCAGTAGCAATC18.333333333333332No Hit
CCAAACTTTGTTACTCGTCAGAAAATCGAAATCATCTTCGGTTAAATCCA18.333333333333332No Hit
AGATCCTTCATAGAAATTTCACGCGGCGGCAAGTTGCCATACAAAACAGG18.333333333333332No Hit
TGAATGCCACCGGAGGCGGCTTTTTGACCGCCTCCAAACAATTTAGACAT18.333333333333332No Hit
GAGAAAGAGTAGAAATGCCACAAGCCTCAATAGCAGGTTAAAGAGTCTCG18.333333333333332No Hit
AAAAAGAAAGGTATTAAGGATGAGTGTTCAAGATTGCTGGAGGCCTCCAC18.333333333333332No Hit
TCTCTTTTTGAGTCTCATTTTGCATCTCGGCAATCTCTTTCTGATTGTCC18.333333333333332No Hit
TTGAACAGCATCGGACTCAGATAGTAATCCACGCTCTTTTAAAATGTCAA18.333333333333332No Hit
CACAAGCCTCAATAGCAGGTTTAAGAGCCTCGATACGCTCAAAGTCAAAA18.333333333333332No Hit
CATCATAGCCAGATGCCCAGAGATTAGAGCGCATGACAAGTAAAGGACGG18.333333333333332No Hit
GATTCGGTACCTTAGCGCTAAAGGTGCCTTTACTTATACCGATATTGCTG18.333333333333332No Hit
CAGGGTTAGGAACATTAGAGCCTTGAATGGCAGATTTAGAACCAGCATCA18.333333333333332No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)