FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s62.352000000d8255.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s62.352000000d8255.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11309
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.1945353258466708No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC451.4451327E-552.6327446
GAGACGT652.4135152E-643.7256668
GGAGACG554.733365E-543.0631527
AGACGTG554.733365E-543.0631529
CGTGTGC504.8344184E-528.4216812-13
ACGTGTG504.8344184E-528.4216810-11
GACGTGT559.2515795E-525.83789310-11
TGTGCTC500.001630097323.68473414-15
GTGTGCT550.002836892821.53157612-13
TGCTCTT1000.00516269314.2108416-17
Produced by FastQC (version 0.11.2)