FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s6.352000000d7b92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s6.352000000d7b92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11191490
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAA380010.3395526422308379No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATG321050.28686975550172494No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATT306480.2738509349514676No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTT284850.25452374974199143No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAA269850.24112070868132837No Hit
GTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAA223910.200071661592871No Hit
ATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAA154440.13799771076058684No Hit
GTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGG137220.12261101962294564No Hit
TTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCC135790.1213332630418291No Hit
CCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTG133880.11962660914677135No Hit
CTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACT129450.11566824435352219No Hit
TTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCC129430.11565037363210798No Hit
CGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATT128690.11498915693978191No Hit
GCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGAC125360.11201368182431472No Hit
AATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAA119220.10652737035014997No Hit
TCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGC118780.1061342144790372No Hit
CTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGG117710.10517813088337657No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC346350.042.6889656
GAGACGT337500.039.741528
GGAGACG364850.039.3920147
AGGGAGA162700.039.204885
AGACGTG321600.038.5015839
TTCCGAT684300.038.437762
CCGATCT681850.038.373874
TCCGATC714150.036.9242483
CGATCTG343450.034.3786855
ATCACTG121300.034.3783765
TCCATCA114750.033.8986852
CATCACT119800.033.6987464
CAGGGAG109050.033.1879924
TGGGAGA148450.032.986045
TAGGGAG44200.032.451624
CTTCCGA851650.032.1171951
CGATCTA153300.031.4467335
GATCTAG60300.027.5678186
CCATCAC145700.027.1542053
ACTGTAC159500.027.0679193
Produced by FastQC (version 0.11.2)