FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s59.352000000d81e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s59.352000000d81e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15268739
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC661700.043.201156
GAGACGT602600.043.1112178
GGAGACG653850.042.4486777
AGACGTG591250.040.4767159
AGGGAGA338700.036.8644265
AGACGTT55900.034.919029
TAGGGAG86000.033.5766374
TGGGAGA290850.033.360415
CGATCTG482400.032.193825
CCGATCT1065050.032.0085144
CAGGGAG216050.031.896664
TCCGATC1129050.030.5306383
TTCCGAT1175500.029.380782
GATCTGG187550.028.8428866
CTGGGAG192300.027.9081634
CCAGGGA117700.025.2598043
CTTCCGA1427950.025.2508091
CGATCTA256350.025.196355
CTAGGGA47450.024.8226153
CGATCTT202850.023.1320445
Produced by FastQC (version 0.11.2)