FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s54.352000000d8149.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s54.352000000d8149.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAATTTACCTCCCATTTAGAGCTCCATTCCCAAACAAACTACTCGTCGA10.6993006993006993No Hit
CCTCTTATGTCTCCTTACACTGCCAGATTAGAGTCAAGCTCAAAAGGGTC10.6993006993006993No Hit
CGAAATCCTATGCAAAGAAAATTTGACACACCGCTATTCAACATACCTAC10.6993006993006993No Hit
GCACACGTCTCCCGTGGGAGATCGGAAGAGCACACGTCTCCCGTGGGAGA10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTACGCAGTCAACAAGGTCAGCTAATGGTCGTCTTATTGCTGTCTCT10.6993006993006993No Hit
AGCTTGCTTGGCCTTTCACCCCTACCGCCCCCTCATCCCAGACTTTTCCC10.6993006993006993No Hit
CGATCCCCGTCCATTTTGCACCGTCGCTGCTCCTCTTGATGTTTACAGTC10.6993006993006993No Hit
CAGCCTACAGTAATTCTCGCCTTCCTACACCAACTTCGAATTCACAAACT10.6993006993006993No Hit
TCTCAGACCCCATCTTCAGATTCGTACCGTGATCCCCCACTCCCCCTTCC10.6993006993006993No Hit
GCATGGGGGTGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6993006993006993No Hit
GTGTAGGAATTCTCCCCGGCCAGTGGTGGCCTCGCCCTCCGCCATTTTTT10.6993006993006993No Hit
AGAGCACACGTCTCCCGCTTTAGATCGGAAGAGCAGACGTCTCGCCATTT10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGCTACCAGTAATATTATCCCGTTTGATGGCGCGAAAAACGCACATCTGA10.6993006993006993No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCAAAATACCAAAACGAA10.6993006993006993No Hit
CTTCTCTGCTCATCTCTTCGCTTGTGTTCTCTTCGCTTCTCTTCACGTCT10.6993006993006993No Hit
CCCATGGAGGAGGGCGACGCTGCTTTCGACGTCTACAACAGGGAGGACGA10.6993006993006993No Hit
GTGCTGATCCGGGATTACTAGCGATTCCGCCTTCACGAGGTCGAGTTGCA10.6993006993006993No Hit
CTTACCGTTCCATTTCATCATCACTTCCATCTCCTACCGTTGCTTCTCTT10.6993006993006993No Hit
GCCGTGACGCGGACGTAAAGAAAAGCAGAGACCAAAGATGATTTCAGTAA10.6993006993006993No Hit
GGCCACAACCTTTAACAAATAGCCACTGCTTATCATGCCGTGAGCCAACC10.6993006993006993No Hit
GGGGAACACACACCACCGTAAGAACGCCGAACTGTAGCTTCTCTGATTGG10.6993006993006993No Hit
CCCGGCACACAGCGACGGTCGAATAGAAGCCCGCGACACAGCACAGCGAG10.6993006993006993No Hit
ATCGAAGACGTTGGGGTCGCCAACCGTCAGCCTCACGAACCCCCCCGACC10.6993006993006993No Hit
CGAGACTAACAAGAGAATCCCCCACCACCCCACTTAGCGCGCACCTCCAG10.6993006993006993No Hit
GTATAGACCGTACCCTAAACCGACACAGGTGGAATGGTAGAGTATACCAA10.6993006993006993No Hit
GATTGTGTGCTTTGGTCTGGAGAATTGAAATCTCAACAAAAAACTACAAA10.6993006993006993No Hit
TCGTCGTGCTAAACTCCAGCCTACACCTACGCCAACCCCCCCACTCCCTC10.6993006993006993No Hit
GCGTTTGCTGCGGGAGGTGTAATTCTTGATAGTTCACACCTGTGATTGTA10.6993006993006993No Hit
CCCTTTCCATTGGTGCTCACATACTTCAACAAAGCATGCAGACGCTAGAA10.6993006993006993No Hit
CTGCAGGGAGACGTGTGCTCTTCCGATCTCTGCAGGGATACGTGTGCCCT10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACAAACCCCGCAATAAGCAGAACCCCAACCAGCTTCCACTCACCCACAAT10.6993006993006993No Hit
CCACTAAAAAGCAATCTTAAGGCCTATGGTGCCGAAACTTAAGTACCCTT10.6993006993006993No Hit
GTCCCTGAACCGGATTACGGTTCGAAGTTAGATATCCAGAGCTGTCTCTT10.6993006993006993No Hit
CTTCCGAACTAATCTGGGGTGCACTTGCGAAAAAAAAAAAACAAAAAAAA10.6993006993006993No Hit
GATCAGCCGAAATGTAGAACCTTATGTGCCGCCAGTCGGGGCGGTACATA10.6993006993006993No Hit
TCATGGACTGTTACCCCCCCCATACTATCCTTGGCTCCGGTTTGCGTCAC10.6993006993006993No Hit
CATTTAGTACACTGCCCATGACATTTCAAATATTTCACTAAATACTCCAG10.6993006993006993No Hit
TGGTGTGGCGAGGTGCTATCATCCAAAGTAGTTCGTGCATTCCTTTGAAA10.6993006993006993No Hit
CACCCACACAGCGCACTCGGGCTTCGATTTTTCCCACTAAAAACAACGCC10.6993006993006993No Hit
GCCGCCGCTTCACACCAGCCCTAGCAACACACCTATTGCCGCCCGTCAGC10.6993006993006993No Hit
GATACTGGACCATCCTTCAATAGGCCCCAAATCCAACCCTCAACTGAGAA10.6993006993006993No Hit
GTATACCACTGGCCGACATCCAACGACCGCTCCAAAACACATTCGCCAAG10.6993006993006993No Hit
GCTACACCTAGACAGACACAGAATGACATAGACCGACCGCGAGGGGGCCT10.6993006993006993No Hit
GGGACAGGATCTCCCTCTTTGTTAACAACCTCTAGGCTGCTAAGTTTCAA10.6993006993006993No Hit
TGCACCAACGACTCCCTTCACTTACAACCTCACACTGATGCCCAATCTCT10.6993006993006993No Hit
CGTCTCCCATGTGAGATCGGAAGAGCACACGTCTCCCATGTGAGATCGGA10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CAAACCTACCAGTTTAGAACGTATTGCGCTAACGTGCCCCCCATATCCAG10.6993006993006993No Hit
TCCCATAACAAGGACCAATTGGGAGGCACTACTGGATCCAGCACGCGCGG10.6993006993006993No Hit
CAAACCCCCAGAAAACCCTCGAACACCAACACATCACAACGAATCCCTAC10.6993006993006993No Hit
GTGACATGAATTGTCCACAACATGCGGCACCCTGTCTATGCCTTCACGAT10.6993006993006993No Hit
AGCAAAACAATACCAACATCACCAAACTTCTTTAGCACTGTGCGTCACAG10.6993006993006993No Hit
CGTCTATTTCTCCTGGCTCCTTCCAACTCCGTCTCTCCCTTCTCTCCCTG10.6993006993006993No Hit
AACTCTTCCACTCCTATAAAACAAAAGCCATCTAATCCCCCCTTCCATCG10.6993006993006993No Hit
CTCATGGGAGACGTGCTGTCACTTATACACATCTGACGCTGCCGACGAAG10.6993006993006993No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAACAAACAACACAAACCAAACAAGA10.6993006993006993No Hit
CGTCCGTACCGCAGGACAATCGGAATCTTGCCTACCAGCCTATTACAGCG10.6993006993006993No Hit
GAAGAGCACACCCGGTTCAGATCGGAAGAGCACCTGTCTCTTATACACAT10.6993006993006993No Hit
CCACTTAAACCTCCCCAAACCCCGGTAAAAAATACACCGCCCATATGAAG10.6993006993006993No Hit
CCGGAGACGCCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGCTTAACG10.6993006993006993No Hit
GTAGTCGCCACGACGTGAAGCGTCGTGGACCGGAATCGTATACGCCTGGC10.6993006993006993No Hit
GTCTAGGGGGCTTATCGGCTTACCGAAATCAGCCAAACTCCGAATGCTGG10.6993006993006993No Hit
TCTGTGTCCTCAACACCATGGTCGGCGGTATTTGCCCTCCTCAGCGGCGG10.6993006993006993No Hit
TGCACTCTCCCACGCACACGCAGCAACCCACAACATTCGTCCAACACGCG10.6993006993006993No Hit
AACCCCACCAGGAAGCGCACCCAACGGCAAACCGCAGCTCCGCGCATCTA10.6993006993006993No Hit
GTCCTGCGGAGCCTTCTCCCCGGGACAAGCTGGTGAGCACGATGGAAGGC10.6993006993006993No Hit
CATGCGGAAGACGTGTGCTCTTCCGATCTCATGCGGAAGCCGTGTGCTAT10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGAACCTAAGAGGTCCATGAAGCCGCAACCGCCGGCCTTTCCGGGTTGC10.6993006993006993No Hit
TACAATAAACCCCTTTCGCACTTCCCCCTCGCATCACCTCGCACCCACCA10.6993006993006993No Hit
CTGCATTTAACCCGTCATCCCATCCTATCCCTCCTGTGCACTCTCTCGCC10.6993006993006993No Hit
ACCCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGT10.6993006993006993No Hit
GCCAACATGTCTGACAGATCCTCCTTTTGCAGGTGATTCTGCTGCTCACG10.6993006993006993No Hit
CTGCTACCTATTAATTTATGCTCATAACTTTATAGTTATAGCTTTTACTA10.6993006993006993No Hit
GCCCAGGGAGACGTGGTCTCTACCGGCCCGCCCCTAACGACCGCGGTCTC10.6993006993006993No Hit
AACGGGCACTGATTGTATTTTTACAGGTTGTCAACTCCTCTTGTTGTGTA10.6993006993006993No Hit
GAATGTGCAGGTACTCAATACGCAGTTGACCCACCACGCGCTGCCAGAGC10.6993006993006993No Hit
GTGCTAAGGTCATTGCCGATTCACTCGTAATACTCTCCAAGTCGGGTTTG10.6993006993006993No Hit
CAACACCCTGCCAGGACCAACGACCATTGTAAGTTGCACAGACCCTATGC10.6993006993006993No Hit
ACTCCAATCAGGGGAAAGGAAATTGGTCCCCGATGATTGGACGGCATCTG10.6993006993006993No Hit
CGGCCGATCTTCTCCGGGGACGACCCCTCCTTTCCGTTACCCGCTGCTAC10.6993006993006993No Hit
CCCGTGCGCCAGCAGCCCTCCCCCCCCCTCCACCCCATGAAAGCCCTCAC10.6993006993006993No Hit
ACGGAGTCAGACCAACGACGCCCCGACACTGCGACACCCAGATTTTTTTT10.6993006993006993No Hit
CTTATACACATCTGACGCTGCAGACGAAGGCTTATGTGAGGATCTCGGTG10.6993006993006993No Hit
GGGCCACCGTAACCAACACCAACCGCCCCTCTACCCTATGCTATCTCAGC10.6993006993006993No Hit
ACTCTGACGCAGCAGGCGGAAGGTTCTAGGTAAATTTCGCGCGTTACTTC10.6993006993006993No Hit
AGCAACACGTCCGCGCAATATTTTATTTGAGAATTCTGTTTTTAACCATT10.6993006993006993No Hit
GCTCCAAATAGACCTTGGGATCGATGGCAGCATCCTTGCTGTGGTACACG10.6993006993006993No Hit
CCTCCATATTACCACCACGGCTATCCCCCCTCCTCTACCTATCCATCCGC10.6993006993006993No Hit
ATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTCTGTCT10.6993006993006993No Hit
GTCCTGGGAGACGTGTGCTCTTCCGATCTGTCCTGGGAGACGTGTGCTCT10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCACCAGAACGAAAACCAGACTCAACTATGCGGACAGTATAGCGACTCG10.6993006993006993No Hit
TCATTCTACCCCACCCCTCAGCTACTCTCTTTTCTAGCCCCAACCCAGCC10.6993006993006993No Hit
GTGTGATATCCGGTAGGAGTTGCATGGTCGGGGTTGCGGGACTTTCCATA10.6993006993006993No Hit
CTCGTCTCCCTGTCTCCACCGCACGGCACCAGACTCTCCTACACATCTAA10.6993006993006993No Hit
CTCTTCCGGGCCGCTCCCCCTACGTCGTCGGTGTACCCTTTGATGAGCAG10.6993006993006993No Hit
CTTCCAATCCAGGCCGAGTACCTGTCCACTGACCAATGTTACTTCTCATG10.6993006993006993No Hit
GGAGACGTGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAATAACCTAT10.6993006993006993No Hit
AGGCCAAACCGATCATGTGTGATATCCGGTAGGAGTTGCATGGTCGGGGT10.6993006993006993No Hit
CCCTGCCAACACAACCCCCTCATGTGCTCTCCCGCGCGCCACTCATTCCG10.6993006993006993No Hit
GGCTAGGGAGACGTAAACTCTTCAACAAAAAAAAAAAAAAAAAAAAAAGA10.6993006993006993No Hit
TACCATTATTTTTATTTTACGTCGCTACCTCCCCGCGTTGGCACTGGGTA10.6993006993006993No Hit
GGCTACGCCAAGAGAGTTACACAGTGAGTAGGACCGCCGTGGGAGCAGGC10.6993006993006993No Hit
GGGACGGGAGACGTGTGCTCTTCCGATCTGGGACGGGAGACGTGTGCTCT10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACCAAACGGCTCAATTAGCGGCTACACAACGATCTGCAGTACTGGAAAGA10.6993006993006993No Hit
GAGATAGTCAATCCCTGCTACTTGTTTATTCGGTCAAACAACAATTCGAG10.6993006993006993No Hit
CCCGACGACCGCGGCAGGACAAAAGAACACGGGCGCTCGTAAGCAACAGG10.6993006993006993No Hit
CCTTGACTCCGAAAACCCTTCTGAAATGCCCAGCAAACATGTTACACTCC10.6993006993006993No Hit
CGTAAACAGCCTCTTCGTGGGACGCCCTGGTTACTCGTCCCTCACTAGAA10.6993006993006993No Hit
CTCTTCAGATCACTGCGGCGAGACGTGTGCTTGTCTCTTATACGCATCTG10.6993006993006993No Hit
GGAGATGGACCCGGAATAGTATATTTGTATGATCGCGTCCGGCACTGGAG10.6993006993006993No Hit
ACCACCCCGGCGGCCGCTGGCGAAGCAAACGGCGACGACGTAAAAGCGAG10.6993006993006993No Hit
ACAAACAAAACACCCAAAAAAAACAGAAAACAAACACACCAAACAAAACC10.6993006993006993No Hit
GCTCGTCCTTTTTTGCCCCCGCTATCTCGCACATCTGACTCTTTATCACG10.6993006993006993No Hit
CCTGCACCAATAAAACAGCCCCACACCACCGTCACCTGGACCGTGGCCAC10.6993006993006993No Hit
GAAGAGCAGCGACGAGCAGAGAAGAGAGAATCAAATACGAGAAGCCAACG10.6993006993006993No Hit
GGTGTTCCTCCTGATATCTGCGCATTCCACCGCTCCACCAGGAATTCCAT10.6993006993006993No Hit
CGGCCTCTCGTCAGTAACAATAAACTCAACAACGCGCCCTGAATGCACCC10.6993006993006993No Hit
CCAAATCCGCATTACGCCCAGAACTCACACATCTCCTACCCTACACGAAT10.6993006993006993No Hit
GTGCTTCTTCTGCAGGTACCGTCACTTACGCTTCGTCCCTGCTGAAAAGA10.6993006993006993No Hit
GAAATAAGAACTGCAACGATCTATAGAAACACTATGCCTTGGAAGTCTTT10.6993006993006993No Hit
CGCACGGGAGACGTGTGCTCTTCCGATCTCGCACGGGCGACGTGTGCTCT10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGACATCTAATTAAAATAAGACATAAGATTTCAAACTAGACCCAATTA10.6993006993006993No Hit
CTTGGAACGTGCTGTTTTGCATCAGCTAGTATGCCTGGAGAGCCACCAGC10.6993006993006993No Hit
GCCACCCCAACGAATGCAGCTCAACCCCATAACAAACCTAAACACCCTAC10.6993006993006993No Hit
GTACGGAAAGTCCCGCAACCCCGACCATGCAACTCCTACCGGATATCACA10.6993006993006993No Hit
TTTCAGACCTTCACGCCCACAGCCCCGCATGCCAGCCATTGCAGCGTCAT10.6993006993006993No Hit
CCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCG10.6993006993006993No Hit
CCTGTGTGCAGTGTGATTTGGGGTGACAGGACTCTGGGCCCTAAGTTTCC10.6993006993006993No Hit
CTGGTCCCCCTTGCAGGTGGTATCCCGGATCACGCGCCTACCTCCGGACA10.6993006993006993No Hit
AGTAGCACCGACCCCTTTGGCAAATCACACAGCCAAAACTCATCTCCGCG10.6993006993006993No Hit
CAACCGACGGTGGTGTCTGATAATCTTATATTCCTGCACCTTAACCTCGC10.6993006993006993No Hit
GCCCACGTCCCAGCAACTATATCCGTGAGTCCTTGTTTGCCAGTATTACC10.6993006993006993No Hit
ATACACACCCTCCGCCGCCTACGACGGCTTACTGTTCCACCATGCAGTTC10.6993006993006993No Hit
CACAACCCACTGAGAGAAGCGTCCCTAAAAGCCCGCGGCACGGCCTGCCG10.6993006993006993No Hit
CGGTGGCAGCGGTGTGACGGAAGGTAGAACAGGTCAGGGGATGTCGGCCG10.6993006993006993No Hit
CCAAGTCAAGAACAACTAGCTCACTCAAACCCCGCCTCCAATCGCTGCAG10.6993006993006993No Hit
AAGGCATCGGAACCCGCCTACTAAGTCCTGTGATGCTAATACATCTCTGC10.6993006993006993No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACCTACCACTACAC10.6993006993006993No Hit
AACCATGATTCCCCGTTACCCGTTGCAACCATGGTAGTCCTAGATACAAC10.6993006993006993No Hit
GGTCTACACATCTTACTCTACCTACGAAGGCCTAAGCCTACCTCCCGGCA10.6993006993006993No Hit
AAAAAAAAAAAAAAAAAAAAAAAAATACCAACCCCCTACACCACACGGGC10.6993006993006993No Hit
CGGGCAAGTTTGGTTCCAGCCCCCCGCCCTGGCGCCCGCGGCCCCCCCCC10.6993006993006993No Hit
CAGTACCAGAAAGACGGAAAGTTGTAGTCAAATAACGGCTTCTTGTACGA10.6993006993006993No Hit
GATACGCCTAGAACACCCGCCGTCCCATCTATCATAGGCACCTGGACGTC10.6993006993006993No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers