FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s53.352000000d813c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s53.352000000d813c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAAGGAATGGCCAGACCCGTCGCTGAGTACCCCGCGCCAGCAATCACG13.4482758620689653No Hit
CACTGAGGCTTCGTTCGCGCTACGCCTAGCAGTAAATGCTTAACCTCGCT13.4482758620689653No Hit
ACACCCCAGACCGCGCCTGCTGTCATATACCCCGCACTTGCCTCTGTAGT13.4482758620689653No Hit
TATGTACTCCGAGTGGAACTATGGACATTCTCATTTGAAGTACCTAAAAA13.4482758620689653No Hit
ATTTAAAAACCTGCATTAGTGCTCTTAAACGAGTGTTATTGTGGGCCGGT13.4482758620689653No Hit
CATTATGACAGAGACCGCTGCTACCGCCACAAAAGACCCGGGGCATGCAA13.4482758620689653No Hit
AAGAGAAGGAGAAGCGGAGTCGCAGCGAGGATCGCAACAAGCTAACCCGT13.4482758620689653No Hit
GTTGTTGCTGCTGCCACTCTTGCTGCTGCCGCTGCATCCGCTGCTGGTTA13.4482758620689653No Hit
AACATTGTCAAATTCATATTTGAACAGTACGTACTCCCATAACATGCAAC13.4482758620689653No Hit
CGCTGGAACAAGAACGGTGCAGTCTCATCTGTCCCAGATTGACGCGAACG13.4482758620689653No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT13.4482758620689653No Hit
TCCCTACGGATGAGCGCAACCCCAACCCAACAACTCACTAAACCCCAAAC13.4482758620689653No Hit
AAACCCAAGTTATACTCAACGGACCGCCACGCCACTAGGACGAGGATCTA13.4482758620689653No Hit
GAACGAAATATTCAAATTCGGGTGCCCGCCCCCGCGGCCGACCGAAACCG13.4482758620689653No Hit
GAACCATCCCCGCGCCCCCCCACGCCCACTACACCCTCACCCATCCCATC13.4482758620689653No Hit
GCCCTACGATGCACCTGCACATTCGTCCGTCCTTGCTCATGTCTTTCCCA13.4482758620689653No Hit
CAGATATTTATCACCTGCACCCCCTTTCGAACAAGTCAACAACTTCCAAG13.4482758620689653No Hit
ATCTATTCAGTTCACGTGCGCGTACTCCATCTTGCACCTAGACTTTCCAT13.4482758620689653No Hit
GCGCTTGGAAGCACGATATGTACGACGGACCGAAGAGGGGTGCCGTCGGT13.4482758620689653No Hit
AGTGGGGAACCATTACCAGATACCACCCCCCCGAGACGCAGCCCTTCCTC13.4482758620689653No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAATTACATACCAACCCA13.4482758620689653No Hit
TTATTCGCAGCGCTGTTTTACCCGACTCTTCTTTTTTTTCACTATGCGAA13.4482758620689653No Hit
GTGTTGTCCGGATACACTTCATTGCTAAAGGAGTGGTAGACGTACATGAT13.4482758620689653No Hit
TATAGTCATTCCCGGTATGCCCAATGCTACCCAACAAGAGATTGCTGACC13.4482758620689653No Hit
GGTGCCTGGCATATCGGATCGGCTGGCTGAGTGGGGCGGGGCGCCTGACC13.4482758620689653No Hit
CTACCGATCCGTAACAAATAATCTCTCACTAGATAACCATCCATCAAAAA13.4482758620689653No Hit
ACATCTCCCCCAGGCAAACCAACAATAACAACAAGCTCCGTTCCTTGCCC13.4482758620689653No Hit
ACACAAGCAAGACATGCAATTGACCCCCCACGCCAACCACCGTGTGTCTT13.4482758620689653No Hit
CCACGTGGAACAACAACGGGCGTACCGCACAACCTGCACCTGGTGCCAAA13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)