FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s51.352000000d8105.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s51.352000000d8105.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTGTGACGTGGACCCTGTGCGGTTGGAGTGTTGTGGGGTCGCAGAGAA13.8461538461538463No Hit
GGCCTGGGAGACGTGTGCTCTTCCGATCTGGCCTGGGCGACGTGTGCTCT13.8461538461538463Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCTCTTCTAGACTCTTTGATGATTGACACCTCTCTACTTATTGTACCGTC13.8461538461538463No Hit
TGAATACCCCAGCACATCTCTGTCTCCACATGCTAGGCACCCGACACCTC13.8461538461538463No Hit
GTCCGCCTCCAAATCCACCGGGTCTGGAGCCTCCACGGCCACCGCGTCCA13.8461538461538463No Hit
GTGTGGACAAGGCCCTGCCCCATCTGGCTTCCAGCCCTGGACAAGGAAGA13.8461538461538463No Hit
CGCAAGTAAGAGCCTCCCAGGTCTTCGACGCGAAACTACCGCCCACGTCT13.8461538461538463No Hit
GTCTCCAGGAGTGAGATCTGAATTTACACAGGAGATGTGAACTGTCTCTT13.8461538461538463No Hit
AGGCGGTACCAACATATCCACTTTCCACAACTAAACAACAGTTCACCAAC13.8461538461538463No Hit
GGGCTATCACCCACTATGGCGTCACTTTCCAGAGACTTCCACTATTCAAT13.8461538461538463No Hit
CGCAGTAGCTATTGAAAACGGCCGCGCGCCACCTATTTACCCCCCAAGCA13.8461538461538463No Hit
ATCAAGAAAGAGCTATCAATCTGTCAAACCTTATTGTGTCTGGACCTGGT13.8461538461538463No Hit
TTTTCTGTCTCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTA13.8461538461538463No Hit
GTGCTCTTCCGATCTGAGCGGGGAGACGCTGTCTCTTATACACATCTGAC13.8461538461538463No Hit
GCACCTACCACACTCTCACCCGCCGCCACACAACCAACACCTGAAACACC13.8461538461538463No Hit
CGCTCTCCAAACCCAATCAATCTTCAACATATATTACACCCATGTTCACT13.8461538461538463No Hit
ATAGTGGGTGATAGCCCCGTACACGAAATTCAAACGGTGGTACTAAGCAT13.8461538461538463No Hit
CAACCGTCATGAGAAACCGACTTGACAACGAACCCACACACAAACGTGTA13.8461538461538463No Hit
AGAGCTAGGCTGAACATCATTTTTCTCAAAATAGTGCACCCGACAGATAA13.8461538461538463No Hit
GGTCGACCTTGAGCGGCGACTCGCAGCCATGTGAAGGTTCGCAGGGTCGG13.8461538461538463No Hit
CATCCGGGCCTGCACAAGCGGTGGAGCATGTGGTTTCATTCGACGCAACG13.8461538461538463No Hit
ACATCACCGCTCACTTTTACCTCTCTCACCCTTCCTACCCCATCCCCCCC13.8461538461538463No Hit
CCCGGGCGGCCCAGGAAACGACCAACCTGCGAGGCACGCGCTCCAATGCT13.8461538461538463No Hit
TGGAGAGACAATTGGCCGTGCTCCAACCTGCTAAACTGTACACAATACCC13.8461538461538463No Hit
ATTCTAGGGGAATCCCTGGGTGGCTCAGCGGTTTGGCGCCTGCCTTTTGC13.8461538461538463No Hit
AGAATGCACAAGGGGCGCAAGCCTGCATCCGCAACGCCCCGTGCCGGATC13.8461538461538463No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)