FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s50.352000000d80f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s50.352000000d80f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGGAATATCTGATGGCCGCTCACATCATCATTGACTTATTGATCTCTC17.142857142857142No Hit
CAACAGAAGAGAATACACTTCCGAAGAAAAGAAAAAAAAAAAAAAAAAAA17.142857142857142No Hit
CCATTGCTAGGCCCCCCCCCTCCCCCCCTCTACCCCGTCACTGTACCGGC17.142857142857142No Hit
CGCAGTAGGAAGTCTACAATACCTCCAAGAAAAACTCTTCAGACTGATTA17.142857142857142No Hit
CACCCGGGAGCTGTCTCTTATACACATCTGACGCGGGCGTCGAAGATGAT17.142857142857142No Hit
AAAAAAAAAAAAAAAAAAAAAATTTTATCCACCCCCCCAACAACAAAAAA17.142857142857142No Hit
AAAAAAAAAAAAAAAAAAACAAAACTCACCCCCCACAGACAGCGCCGAAA17.142857142857142No Hit
CCTGAGGGAGACGTGTGCTCTTCCGATCTCCTGAGGGAGACGTGTGCTCT17.142857142857142Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAAAAAAAAAAAAAAAAAAATATATAATCCCCCCCCCAACACAAAGAA17.142857142857142No Hit
ATCGGGGGGGCACGGGGCGGGCCGGCGCACCCAAGTGGGGTTGGGGAGGC17.142857142857142No Hit
CTCTTATTTACCGCCTACTGCACAGTGCACCTTACCCTCCGTTCGCGATT17.142857142857142No Hit
TCGACGCATAAGCGCAACCCATTGGTCCTCCGATGTGTTAAGACTTACCA17.142857142857142No Hit
CGCCTCTCTTGATACCACTATACCCCATCTCCACCCCACGCGCTTTGCCA17.142857142857142No Hit
TAGAGAACTAAAACGAGCTGAAGACAAAGTACAGGACCAACCGCCAACGC17.142857142857142No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)