FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s5.352000000d7b6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s5.352000000d7b6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9626627
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTAACACCGTCCGATCCCTAGTCGGCATAGTTTATGGTTAAGACTACG471590.48988082741753686No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAA284730.29577337939862014No Hit
GTTTATGGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCCCTAACT230570.23951275976518047No Hit
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTACGTAT191260.19867810397141178No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA191190.19860538899034935No Hit
GAATAATAGAATAGGACGTGTGGTTCTATTTTGTTGGTTTCTAGGACCGC160450.16667312444950863No Hit
GATTAATAGGGATAGTCGGGGGCATCAGTATTCAATCGTCAGAGGTGAAA159770.1659667503477594No Hit
GTGTTAGTATTTTGACCCGTTCGGCACCTTACGAGAAATCAAAGTTTTTG150690.1565345785185195No Hit
GTATTCAATCGTCAGAGGTGAAATTCTTGGATTGATTGAAGACTAACTAC144610.1502187630205263No Hit
ACCATACTCCCCCCAGAACCCAAAAACTTTGATTTCTCGTAAGGTGCCGA142710.14824507067740342No Hit
ATACTAACACCGTCCGATCCCTAGTCGGCATAGTTTATGGTTAAGACTAC130250.1353018040482923No Hit
GTGCCGAACGGGTCAAAATACTAACACCGTCCGATCCCTAGTCGGCATAG127450.13239320480579542No Hit
GCTCTAGTAACGGCGAGTGAAGCAGCAATAGCTCAAATTTGAAATCTGGC126060.13094929303898448No Hit
GAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCT125100.12995205901298557No Hit
CTCTATGACGTCCTGTTCCAAGGAACTTAGGTCGGTGGCCACTCAGAAGC118520.12311685079311788No Hit
ATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATTCCCAA115400.11987584020862135No Hit
GTATCTGATCGTCTTCGATCCCCTAACTTTCGTTCACTGATTAATGAAAA112460.11682181100399963No Hit
GGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCCCTAACTTTCGTT110010.11427678666681487No Hit
GAATAGGACGTGTGGTTCTATTTTGTTGGTTTCTAGGACCGCCGTAATGA108210.11240697286806686No Hit
TTCCCAAACAACTCGACTCGTCGAAGGAGCTACGTATAGAGCGCCTTTCC106850.1109942246645684No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG260000.051.4396175
TTCCGAT577700.050.842
CCGATCT588000.049.707124
CGATCTA114800.043.9360545
CGATCTT99750.042.6612475
CTTCCGA717000.042.419991
GATCTGG117500.041.996796
GATCTGA72800.039.3390926
GGGAGAC180600.039.3153276
TCTACTT110450.038.5713772
CGATCTC176600.037.4357835
TACTTGT117150.036.405964
CTACTTG117700.036.1551323
AGGGAGA89450.035.6802065
TTGTGCG120600.035.1282047
GATCTAG45450.035.1110536
TGTGCGC122900.034.548098
GGAGACG203500.034.120977
TCCGATC866300.034.034693
ACTTGTG125300.033.924265
Produced by FastQC (version 0.11.2)