FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s49.352000000d80dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s49.352000000d80dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCGGGAGACGTGTGCTCTTCCGATCTTCTCCGGGAGACGTATGCTCC111.11111111111111Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATACGCTACGCCTGACATTTATACCTTTGTAATATTCCGTTTGCTTTTGC111.11111111111111No Hit
GTTCAGTTCGCAGCGACCGCTGGCCAGCGTGCCAGGTGCACACCCGCAAT111.11111111111111No Hit
CGCGTGATGAGCGGGGAAAGAAGGCTATGTTGAGATTGAGTGTCGTTTGA111.11111111111111No Hit
CCCCGCCTTACTACCAGCAGCAGGCCAGGACACACACACACGAAACTCAC111.11111111111111No Hit
ACCCCGGGGCTGTCTCTTATACACATCTGACGCTGCCGACGAATAGAGAG111.11111111111111No Hit
TTCGAAGTAACCGATCTTGCCTTAAAGCTCTTCCTGACACCAGCTAGCCT111.11111111111111No Hit
CATCGAGACTCTCACCGTCAACCACACACTTCTCTCGGGTCTCATCCCTG111.11111111111111No Hit
GTAGGATTATGTCGAGCGGGGAACTGTGCTTGCTGGCGCATTGCAAAACC111.11111111111111No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)