FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s46.352000000d8070.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s46.352000000d8070.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7942110
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83930.10567720668688799No Hit
GTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAG81030.10202578408004925No Hit
ATTATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAAT79450.100036388314944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC411050.042.0691646
AGGGAGA182300.041.762335
CAGGGAG102000.041.1613854
GGAGACG408700.041.1606067
GAGACGT383450.040.7621468
CGATCTG340900.039.3019375
AGACGTG372450.038.484799
CCGATCT760650.037.132224
TTCCGAT796500.035.8246542
TCCGATC808800.035.3385623
TGGGAGA156150.035.2212265
GATCTGA80250.034.9176376
GATCTGG141300.034.217126
AGACGTT36900.032.8209889
CGATCTA150200.030.3872435
CCAGGGA58400.028.7891123
CTTCCGA1072950.028.559641
GATCTCA65650.028.0695136
TAGGGAG59700.027.5257784
CGATCTC234800.027.408115
Produced by FastQC (version 0.11.2)