FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s35.352000000d7eef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s35.352000000d7eef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length101
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACACCATAGACGGCTTTCAAATCCACCATAACCAGCCCACTCACTCTCC11.2048192771084338No Hit
AGAAGCAGGGCCACAATACCCAATACAACAACAGTTCTCGGGGCCCCACT11.2048192771084338No Hit
CACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCAC11.2048192771084338No Hit
CGCGCATCACATGACGTCGCCCGCAGCGACGGCCCCACCCATCCCCAGCG11.2048192771084338No Hit
CCGGGCCGATCGGCACGCGACCGCCTAACCAGCACTCGTGAACACATTGA11.2048192771084338No Hit
AAAAAAAAAAAAAAAAAAAAAACAAAACCAACCCACCCACCAACCCCCCA11.2048192771084338No Hit
TAATGGTCGCCACTGTTGTCGTTCGTATTTTGTACGCCCCGTAAGAACTT11.2048192771084338No Hit
ACGTGCGCAGACCATCCACAACTCCGCAGCACACTCGCCGTAAAAAGCTC11.2048192771084338No Hit
TCTCTCCCACAAAAAAAGCACCCCCCCACTAACCTCTCCATTACCCGGAC11.2048192771084338No Hit
AACGTCCCTACACTCCCACCGAAATCCTAAGGACACCCTCCGTTACCCCC11.2048192771084338No Hit
AGGACCATGATGCGTAAGCCATCCCTTAAAGCCGTAGACATGAAGTCGAC11.2048192771084338No Hit
CCACCCAGAAACCACCACTCGAACCACGCTAAGACTCCCAGGAGACCCCT11.2048192771084338No Hit
ACGACACTCCATACACATCACCATCCCTCGTCCCGCTAATCCTCCATGTT11.2048192771084338No Hit
AACATCAAAGACTCCCCACAATCCCTCCTCTCCTGCAGGATTCACTCACC11.2048192771084338No Hit
AAAAAAAAAAAAAAAAAAATAAACAAAAATCACCCCCCCCCCCCCCCCCC11.2048192771084338No Hit
CACAACGGAGCCCGAAAAATGTTCCTAATCAGCCTCGCGAACGTCTGTCA11.2048192771084338No Hit
TGCCGCCCACCGTATGCGGGATACCCGCCGGGGGGGGCCGGCCCGGCCCC11.2048192771084338No Hit
GGCGCCGAGCTTGAGCGCAGACATCTGCCACGCCTCGGGTTCGTAGACCA11.2048192771084338No Hit
AAGAGACCTGCACGGTCTTGGCCGTGACCCACCACACATCTCCCCAGGGC11.2048192771084338No Hit
CCCAGGCGAGAACACTAGCGAAGCCATATCGCGTAGGGAGGCTGCTTGAA11.2048192771084338No Hit
CCCACTTGACAATTCTTTCGCTCCTTCTCTACTCTATCCAAGTACCCGTC11.2048192771084338No Hit
CCCGGGCAACCACAACGTTGCAGGACCCACAAAGTATTTGGATCCCCGGA11.2048192771084338No Hit
TGGTGGGGAGACGTTTGCTCTTATACACATGGTGGGGAGCCGAGTGCTCT11.2048192771084338No Hit
CAAGATACAAAAAGAACGTATACGATGGCAGTGCCTCCCAGACACGGTGT11.2048192771084338No Hit
GTCTTAGTTAGTAGACTTTGATAGCAGTGACGCTATGTGGTTTCCAAAAT11.2048192771084338No Hit
ACACCACTTCCCATCGTAAACCTACTCTACAGTTACACTGATATGGCGCA11.2048192771084338No Hit
AGAACAGCCTGCAGTGCTCTGATCCCACCGCGGCGCGTGACGAGCGCTGC11.2048192771084338No Hit
TGTGTGGACTGTCTGGTAGGTAAATCCGCTGGGCGTGAAGGTTGAGGCAT11.2048192771084338No Hit
CCCTCACCTAGATCTCAGTACCCTCGAAACCTTACAGGGGCGACGCTGGG11.2048192771084338No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA11.2048192771084338No Hit
CCACGCACCTCGCAACCCCCCCCCCCCCCCTCACCACACCCTACCCCCCC11.2048192771084338No Hit
CCAACCATACAACAAAAACAATACTAACAAAACTCTACAAACTCCACACT11.2048192771084338No Hit
ACGACATTGGCACGCCCGGACATCTCCTCCGACCGCGCGGGCTAGGGGCT11.2048192771084338No Hit
GGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATAAGCTGCGGGGA11.2048192771084338No Hit
CGAACCTTACGCCACAGGTACTACTTCCCCACCATAAGTAGATACGAATG11.2048192771084338No Hit
CAAAGGCTCCACACGGCGCCAATACCCCCAACCGGCACATGAGACACGCA11.2048192771084338No Hit
CTGACGGGAGACGTGTGCTCTTCCGATCTCTGACGGGAGACGTGTGCTCT11.2048192771084338Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCACACGACCTAAAACTCCGCCCCCCCAAATCGTATCCAGCCCCCTCTC11.2048192771084338No Hit
AGCGTTGCAATTACGTTCCCCCTCCGCCCCCTCGCCTCGTTATCCCTCGT11.2048192771084338No Hit
AGCGATTTCCCTTAATCACTCTACATTTTTTACACTTCCGTAAAAAACCC11.2048192771084338No Hit
AGCCCCCTAGCCCGCAGGAGCCCCTGTTGCTCCGCGGCTACTAAATCCTT11.2048192771084338No Hit
AAACTGGGAGACGTTTGCTCTTCCGACCTAAACTCGGCGACGCTTACCCT11.2048192771084338No Hit
CACAACTGACCCTTACATCACTTGTACCCCGCTGCGTTGGATATTGATTA11.2048192771084338No Hit
CGGCCTCCACAAATATTCTACCTCCCTCCACTCCTCGTAATAGTTACCCC11.2048192771084338No Hit
TGCCTCATACAGAACCTGGAACCCCGATATGAAGAGCCTAGGCATCCAGT11.2048192771084338No Hit
CAACATCCAAATACTCATTCTTCCCTTTCCCGTCTAACCGCCCCCCCGAA11.2048192771084338No Hit
TGCCAGGTCCGGCATTTGAGGAAGCCGGCTTGAACTTGGCATTCACCGTG11.2048192771084338No Hit
ACGTCCCAACCCACCAACCAAATCCCCTGCTACGTCTACCGACCCAGTGC11.2048192771084338No Hit
AGTAAACATGAATCCACCAATTGCTGCGCTACAGCTCACGGTGTGCCAAC11.2048192771084338No Hit
CAATTACAGGGCCTCGGATATGAGTCCTGTATTGTTATTTTTCGTCACTA11.2048192771084338No Hit
ATGACAGCAACTTCTGCCCAGTATAAAACTCGCCGGGGGCTCAAGGCTAG11.2048192771084338No Hit
ACACCCCCGCGTATCCCACACATCCGCTCGCGCGAACGTCCAAACTGCGA11.2048192771084338No Hit
CGCACAGTGACTCAGGGGCTAAACAGAGATTCCGTCGGCGGCAGGTAATG11.2048192771084338No Hit
TGATACTGCCGATACACTCATACAGTAACATGTAGCGCGGTCTGCTACTA11.2048192771084338No Hit
CGAGACGTTAACCGCCCACTAAACGATATCTTAGCACTAGAATCAACTAC11.2048192771084338No Hit
TATGCACATGTTAACCCTACCTTACCCACCAACAAGTCCATCTTCAACTC11.2048192771084338No Hit
CGACCGGGAGACGTGTGCCTGTCTCTTCTACACCGCTGACCCTGCCCACT11.2048192771084338No Hit
TCCGCCTGACTCGAATCTAAGTCCTGGTTCATCTCCCTCCCACCCTCACG11.2048192771084338No Hit
CGCCCAACCCCAGATCTTTCGTCGCCCACAGGCAAAGCAACCCGAATGCG11.2048192771084338No Hit
AAAAAAAAAAAAAAAAAAAATATTCTCCACACCCCACCCCCCCCCCCCCC11.2048192771084338No Hit
ACCACCTATTTCTTATCGAATATTACTCACTTTGGTCCTGGTATCCATAT11.2048192771084338No Hit
CTGCAGGGGTCCTGCATCCCGCTCCCTGCAGGGAGTCTGCTTCTCCCTCT11.2048192771084338No Hit
CACCCAATCAGAGCAAACGCGGCTGTCTGTTCCCACAGTTGCACAAGCTT11.2048192771084338No Hit
CCCGATCCATACTACCGCACCACTCCGACAGGGCGGGCCTGTCGGCATAC11.2048192771084338No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAGAAAAGCAAGCACAA11.2048192771084338No Hit
AAAAAAAAAAAAAAAAAAAAAAAAATACAAAAACCCCAACCCACACACCA11.2048192771084338No Hit
CGCAATGTTGAGAATGCGAATCTTCGGACACCGGCGTGTGCACGCAAGGA11.2048192771084338No Hit
CCCCCCCCAATGCACCCCCCCAAACTCACCCCTCGACTCGCTTCACCCCT11.2048192771084338No Hit
ACCTGCCTGACCTCACTGATTCCCATCAACCCCGTATCCACAGCACACCC11.2048192771084338No Hit
AACCTTTCCCCCCAACTTCCCCTCCACCACACACACACTAAGAAGAATTA11.2048192771084338No Hit
CACGGGCCCATATTTAAAACACTCCTCCCCCCCCACCAGGCCAGCAACAG11.2048192771084338No Hit
ACCGCCCCCGAGAAAGCAGGACACATAAACGTCTCTGCGGCTGCCCGATC11.2048192771084338No Hit
CCCCAGGAGACGTGTGCTCTTCCGATCTCCCCAGGAGACGTGTGCTCTTC11.2048192771084338Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCACCACGGCACCAACGGCAAACCTATACACATCACCCAGGTCCACGATG11.2048192771084338No Hit
ACAGCCCTAGCCCATGCACCATCTTCGCCAGGAGTCGCGATGTCAGACGC11.2048192771084338No Hit
CGCGGACTAACATATCGGGGCTCCCGCCGCGCGAGCCGCTCGCCTCACGC11.2048192771084338No Hit
CGAGCTGTCCCCAATCAAAGTAAGAAGACCAACCCCTACCCATCACATAT11.2048192771084338No Hit
ATCTCCCCGGACACCACATATGTCCGGCACCGGCCAATCCGAGTACCGAC11.2048192771084338No Hit
TCAACACTTCAAGTACAACGACCCCACGTGCATCGCGACACGCGGCCAGA11.2048192771084338No Hit
GGCCACCCCAGCCCCGACTTCACCTCGCCACCGTCCCTTCACCCTCTGTT11.2048192771084338No Hit
AAAAAAAAAAAAAAAAAAAAAACAAAACCACCACCACCCCCCCACCCCCA11.2048192771084338No Hit
AGCATCCCAGTATTGCCCGTCCTACACGCGACGACTGCCGGGGCACCCCC11.2048192771084338No Hit
CTATAATAAGAACGCTTGAACGACCCTTGTTCCAGCGATTTATGAGGATA11.2048192771084338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTG50.094.999998
TTCGTGT50.094.999995
TGTTTGC50.094.999999
TCGTGTT50.094.999996
ACTTTCG50.094.999992
CACTTTC50.094.999991
CGTGTTT50.094.999997
TTTCGTG50.094.999994
CTTTCGT50.094.999993
Produced by FastQC (version 0.11.2)