FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s34.352000000d7ec5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s34.352000000d7ec5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACACTAGAAACGACTAACTGTTGCGGAACCGACCGCCATACCTCTCCCT11.36986301369863No Hit
AGGAGCTAGGTACATGCGAGCTCCATCACGGATTCGACCGTATGCATAGT11.36986301369863No Hit
AAAAAAAAAAAAAAAAAAAAAAAATAATTTTTCTTCCACCCAACACGACC11.36986301369863No Hit
CATTGTGAACCTCTCTTTCCCAGCTCCTCAGAAGTCGTCAAATGGCTCCC11.36986301369863No Hit
TAAACGCTTGACCCGGTCCATCTAGAACCTCTGTGCGGCGCTGTTCCGCC11.36986301369863No Hit
CGGGCCCCAAACCCCCAACCACTGCTCAAGCAGTTCCTCTTGGGGCCACT11.36986301369863No Hit
CGCACTCGTTACCCACCCTCCACCCTCCCTCCCATGCCGCCACCCGCAGT11.36986301369863No Hit
GCCCTCGGATACCCCGCCCATTCTCCCAAACAACCCCTTCTCCTAACACC11.36986301369863No Hit
AAAACGGAAAGAAAGCAGCAAACCGCCGCAAACGACCACACGCAAACCCC11.36986301369863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAATATAATATCCCCCACCACCAAAGA11.36986301369863No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAACACAAAAAGAAAAG11.36986301369863No Hit
TTGCGAATGCCCCCTCCGGGACCTGTCATCGCAACACCTCTAACTATTTT11.36986301369863No Hit
AACGTTGGTGCATTTGTGCACCGGTGAAACGCAGCTGTCGTCGCGGGTAA11.36986301369863No Hit
CCTACAACCGACCCCATAACGTATCTGCCCCACTGAGCCATAATCCCATC11.36986301369863No Hit
GGTCAGGGGTGCTGTCTCTTATACACATCTGACGCTGCCGACGAAGAGGA11.36986301369863No Hit
TCAATCCGTCCTATGTAGTTTCTCCACCGCTGCATTGGGGGTACCCCTGG11.36986301369863No Hit
GCTTGTATCGTTGTGGTCATTTTTAATTTTAATTTCTTGATCCAGCAATC11.36986301369863No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCACAAAAACAAAACAAA11.36986301369863No Hit
GGCCAGGGAGACGTGTGCTCTTCCGATCTGGCCAGGGAGACGTGTGCTCT11.36986301369863Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGAACACCGGCGCCCGAATTTGAACGCACCTACCCTGGGGGCTGGCGGGC11.36986301369863No Hit
GCACTGGGAGACGTGTGCTCTTCCGATCTGCACTGGGAGACGTGTGCTCT11.36986301369863Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTACCGGCCAACCTAGCCACACACACCCAACATCTCCATCAGACCCAA11.36986301369863No Hit
TAAAGTGGTAGAGACCCCCGATCACCGCGCACGTTCAGCTTTGCCTCTTT11.36986301369863No Hit
CAATTAATTAATTCCGCAACCACAAAACGCAATGTAAATGTTTAAATATC11.36986301369863No Hit
TCCGAGGGAGACGTGTGCTCTTCCGATCTTCCGAGGGAGACGTGTGCTCT11.36986301369863Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CACAGAAGCACGACGGAGACTGCCAACTATCCCACACTCAATGTAACCCC11.36986301369863No Hit
CACGGGTTAGACAAATAACCGCACCACCCAACCAGTCCGCGCTCAGAGCT11.36986301369863No Hit
TATTTATATTATTTATGCCTCTAACTGGAACGTACCTTGAGCATATATGC11.36986301369863No Hit
CCTCCTCGTTTTCCCCCCCTCGACCCCCCACCCGAAACTGAACACACCTC11.36986301369863No Hit
ACTCCGGGAGACGTTTTCTCTTCCGAACTACTCCGGGAGACGTGTACTCT11.36986301369863No Hit
GTAGTCCGTTCTCATTGGGTTCCTCTTTAAGCCCCAGGACACTCAGACCT11.36986301369863No Hit
TACGGAACTATCCTAACCCTCTCTTGGACTCCATCTCACATGTTTCCACA11.36986301369863No Hit
CAACAACGCTACTTATTCTATTCCCACCCCCCCCCAACTCCGGCGAGGGG11.36986301369863No Hit
GATTTGTATTTAGCCAAAATTCATGTAAAGGTTTACTAAACCTGTCTCTT11.36986301369863No Hit
TCTCTGGGAGACGTGTGCTCTTCCGATCTTCTCTGGGAGACGTGTGCTCT11.36986301369863Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AAGAAACAGGTAGACGAACCCATCCAACTTGGCCAATGACTTCTTCCAGA11.36986301369863No Hit
GGGTGGAGACAACCCCAGCTTTACCTCAAGCCGATGTAAGTCGAACGAAG11.36986301369863No Hit
AGGGACATTCACTCTAAAGACGTCCAAGCAAGGTAGCCTACTCACACACA11.36986301369863No Hit
AACAAGGAGTGCAATAACAGTTACACGCTGATAAGACTGTCTCTTATACA11.36986301369863No Hit
CGGGGGCCGACGGTTCCCCAGAATACCTCCCCTACCGCGACCCTGTGAAC11.36986301369863No Hit
CAGGCTGCACTGGTCAGGAGGTCTATAACGAGGGCAGGACCTAACTGTCA11.36986301369863No Hit
AAAAAAAAAAAAAAAAAAAAATTTTAAAACCCCACCAAGAAGAAAGAAAA11.36986301369863No Hit
CAGCTGGGAGACGTGTGCTCTTCCGATCTCAGCTGGGAGACGTGTGCTCT11.36986301369863Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAACTGGGAGACGTGTGCTCTTCCGATCTCAACTGGGAGACGTGTGCTCT11.36986301369863Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCATGTCTCTTAAGCCGAAGCTTTATCCGCACCCGCTGCTCTTTCTCAAT11.36986301369863No Hit
CAGACCGAAGCACTTTCTGTGGTGTCATAAGACAACTATCTGTCCAGTAA11.36986301369863No Hit
TCAACCCCAACACGAAAACGCAACCCCGCGAGCATCGCCATCAAATCGCC11.36986301369863No Hit
ACCGTGGAGACACCGACTCCGATAAACACGGGCACGGCGCGCTCGGGCCG11.36986301369863No Hit
CCCACCTATTCACCACTTCGCCAGCCTCTATCTGCTCTTTTTAACCTTTA11.36986301369863No Hit
CCCCCCTCAGATCTTAACGACCTCCTTCTCCACCGTGCGATCAAACACGC11.36986301369863No Hit
ACCGGCTTTTAGCTATCCGCGTGCACAGATCCTCGTTACACCCCGCCCCC11.36986301369863No Hit
GAGCCGGGGCTCTTTTGTATTTCCGTCTGACGCTGCGGACGAAGAGGATA11.36986301369863No Hit
TGGGCTTAAACATTGAACCGAAGGGAGAATACCAGCGTGAGACCGAAGTG11.36986301369863No Hit
CCCGCTCCCCATACTTTTGACACCACACATCTAGCAGCTGATACCCGTTG11.36986301369863No Hit
TGAAGTGCGTGTGTGTGTAGGGCTTGCGCGGCCCCCGATCCCCGTCTTTT11.36986301369863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACATAATCATTTTACTCCT11.36986301369863No Hit
ACGCGCTCAGCAACTACCGTGCTACGTGAAGTGCGCATCAATGAGACGTG11.36986301369863No Hit
TGAGAGGGAGACGTGTGCTCCTGTCTCTTATACACATCTGACGCTGCCGA11.36986301369863No Hit
GCTTTCACCCCCGACTTAAAACGCCGCCTACGCGCCCTTTACGCCCAGTG11.36986301369863No Hit
GGACACGGGGAATATTAGGGCAGCATAACACACCCATTCCGATGGGCATA11.36986301369863No Hit
GCAACTGGAAGTCAAGACCGTTACTTCAGACGCACCGTGCCCTATTAGAA11.36986301369863No Hit
CTCAATACCCAGCCCGCCTCATCCTCCCATCGCTTCTCCCTCAAATCTCA11.36986301369863No Hit
TAAGCTTACTTGGCCTCCCTTTCGCTTTCTCCAAGCCCAGGTGTGTGAAC11.36986301369863No Hit
CCTGCTCCGCTACATCAGACCCCCCGCGCTCACGTTTTTGGAATGTTTAT11.36986301369863No Hit
ACGGCTGTGCTATAACCTCACTTCTTCCCCCACCCCCAACCCGAACCCCC11.36986301369863No Hit
ATAGCCCGAGCTACAGAAAGCAAGTGCCAAAACACGCCCTGCCTCTTAAA11.36986301369863No Hit
GATGTTATTAGTACGAAAGAAATATAAAGAATACAAAAGTACAGGTGGAC11.36986301369863No Hit
TTTTCTGTCTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTA11.36986301369863No Hit
GGCGAGGGAGACGTCTCTCCTGTCTCTTATACACATCTGACGCTGCCGAC11.36986301369863No Hit
AAGCATCGGGTGTTCCACGTGTCGTCCACTTGCTAATTATTTTCGACTAC11.36986301369863No Hit
CGGCAGGGAGACGTGTGCTCTTCCGATCTCGGCAGGGAGACGTGTGCTCT11.36986301369863Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCTGGGACTGTCTCTTATACACATCTGACGCTGCCGACGAAGAGGATA11.36986301369863No Hit
CAGTCTGTGTAGCCCGTATAGCTTTAACGATTGCTGTCCGTGAAATCTCC11.36986301369863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCAAC50.094.999998
CGCTCAG50.094.999994
TCAGCAA50.094.999997
GCGCTCA50.094.999993
CTCAGCA50.094.999996
AGCAACT50.094.999999
ACGCGCT50.094.999991
CGCGCTC50.094.999992
GCTCAGC50.094.999995