FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s33.352000000d7eb8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s33.352000000d7eb8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72
Sequences flagged as poor quality0
Sequence length101
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTAGAATTACACCGGTTATCCGAGCAGCGCCGCCCATCAAACAAACT11.3888888888888888No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAATTATTCCCTCACCCCAAAACAG11.3888888888888888No Hit
ACGCAGCGAGAGGTGGGTCTCCCTCCACCTGATCTCGACAATGGACTCCA11.3888888888888888No Hit
AGCGGCCCGAAAAGCTCCGCCTACCGTTACCCTGCCGCCGCGCGGATCCC11.3888888888888888No Hit
TCTTAATCCAACATCGAGGTCGCAATCTTTTTATCGATATGAACTCTCCA11.3888888888888888No Hit
CTCCAGGGAGACTTTTGCTTATACACATCCTACGCTGCGACCGAATCGAG11.3888888888888888No Hit
AAGAACCACCCACCCACACTCGTCCGCAAAAAACCCACATTAAAATATAC11.3888888888888888No Hit
AAGGCTACAGGACCGAAGGGGGCTCGCGTAGAACGTGTGATCGCGCTCAC11.3888888888888888No Hit
TCAAGGCGGACCGGGTGTATGTGGAGGAGTGGGGCTACCAGGGGCGGCGG11.3888888888888888No Hit
CAAACCCATCTCCCCTCAAAGAACTGAAACGCCGTATCCCATACCTGAAC11.3888888888888888No Hit
TCAACAACAAATCGCCAGACCTCTCGAGAGCACAGCGAGCGACCTACCAC11.3888888888888888No Hit
CGTTATCAGGTTACACACATAAGCAAGTATACCGCCCCTCCCCAGTCTCA11.3888888888888888No Hit
TACCAAAATCACCTTTCGGCGCCCCCCCCACCTCCATACACCACCCAAAA11.3888888888888888No Hit
TAGGCAATACTGAGTATGCTGCGTCCACTCACACTAGCTCCACGACATCG11.3888888888888888No Hit
CACACCCCGGAACACACCCCCTAACAACCCGCCTCCGCCCCACTTACCCC11.3888888888888888No Hit
GCACGAGCAATATTCACCCTCATACATACTCTCAGCGGATAAGAGCGCAA11.3888888888888888No Hit
AAACGTCCTGAAACAGAAGGATTTTCAACCAGAAGTGATGAGTCGCGACT11.3888888888888888No Hit
TAGACCACTGAAGGTTCGCCAACCAGGTCCATGCCTGTGGGTCAGGAGTC11.3888888888888888No Hit
TGTGGCGCCATCGACGATTGCCGGTTGACCTGGGGCGTCATATTGATACG11.3888888888888888No Hit
CAAACCACCATACACACCACCTCTCCAACTCCGAGGCCATCCTCTACCGA11.3888888888888888No Hit
CCCACACCACCGTCACCTCACCCCCCCCCCCTCCCTGGAATGCCCTGCGA11.3888888888888888No Hit
ACAAGGTAACAGGTCAGGTCCACAAGTCGCGTGTACGGCAGGAAAAAACG11.3888888888888888No Hit
AAAACAGCAATCAGTATGTAGACTCTCAGTGTCGTCGCGCTTACGCTTGC11.3888888888888888No Hit
AGGCGCCGTGAAGGTCCCCCAATATGACCCGTGTAGGACCGCGGGGCCGC11.3888888888888888No Hit
TAAAACCTCTCAAGGAACATCCGCCCATCTGCACGAAATTCACTCTCAAA11.3888888888888888No Hit
GAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAG11.3888888888888888No Hit
TCGAGATAAGTACACACCCACCTAACCACACTACATTGGGCATTCCAATC11.3888888888888888No Hit
TAAAGCCCTCATGTCAATCTTACAAGACCTCTGACAACCTAACACTTCTC11.3888888888888888No Hit
AAAACTCGAGATAGGCCAAAGATCAGAGCACGCGCAATAATACCCCCACC11.3888888888888888No Hit
CAGATATTAGAAATCAACCAGGACCCAAAAGGCCGGTATACACTCAGGCG11.3888888888888888No Hit
TGGTTGGGAGACGTGTGCTCTTCCGATCTTGGTTGGGAGACGTGTACTCT11.3888888888888888Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TGTGATACAAAGAAACGAACGAACCTTCCATTAAACCACACCCGTACATG11.3888888888888888No Hit
TACCGACGTCGCCTAGGTGACCCTCTAACGCCACGCTCGCCCCCCCAGCC11.3888888888888888No Hit
ATCGCATTCTAAACTACGGCACCGCCGACGCCTGCTCGTAAACGGTGCCG11.3888888888888888No Hit
CCCCCTAATTCTCCTGGGACCGCGGCCCACTCCGGCGGCTGCTCCCCACC11.3888888888888888No Hit
ACCTTGGGAGACGTGTGCTCTTCCGATCTACCTTGGGAGACGTGTGCTCT11.3888888888888888Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACCCTATAACCCGCCCCACTCCCCATCTCCACACAACCCAACTATCCCC11.3888888888888888No Hit
ATGGGACTAGTCAGGGACCCCTTTCACTCAACTATGCTGAGACGTTCATG11.3888888888888888No Hit
TACCGCGCTCCCCTCCTCCCCCGTCCGGGCCCGCCCTCGCCTCACGCCCG11.3888888888888888No Hit
TTACCCCCCACCGCCCTCTCCCCGCCCTGGCCCCGCCCCGCCCCCTGCCA11.3888888888888888No Hit
AAAAAAAAAAAAAAAAAAATATATTCACCTACACCCCCCACCAAAAAAGA11.3888888888888888No Hit
GGGAGACTTCGGAAACAACCACAGCGCGCGCCTTCACCCATCACTCAAAC11.3888888888888888No Hit
GGCACGGAGACGTGTGCTCTTCCGATCTGGCACGGAGACGTGTGCTCTTC11.3888888888888888Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTACCTCGGATAAAACTTCCCTCCACCACTCCCCCTCCCAAACCTCTGTC11.3888888888888888No Hit
AACAATCCCCTTCCCCCACCATCCCTCCTACTCCCTTACTTTAGTTCCCC11.3888888888888888No Hit
AGACGTTAGAGGTACACGCGCAAGCCCCGCGCGTCCACCAGACCGCCCCG11.3888888888888888No Hit
ATACCCTTTTCAACTGAGTTATTCCCAACAGCTCGGGGCAGTGACCGAGC11.3888888888888888No Hit
CATCGCACAACGCTCCACCCAGCCCGAGCTGCGCGTTGAACACACCCCCC11.3888888888888888No Hit
TATCGCGAAGACCCCTAACCTCCGTGACCTCGTTCGCCACACTCCCCCCA11.3888888888888888No Hit
AAGCGCGGGCCGCATCAGTAAAGCCTCGCGCCCGCGGGGCAGCCCCACCC11.3888888888888888No Hit
AGGATTGCCTGCAAACGGTCCTCGAGGTGGTTGAGCTGGGCATTTCGGGC11.3888888888888888No Hit
TTTTCGATCAACCGCTCTGAGTTCATATCGGCTTCCATTTCAAAGAGCTC11.3888888888888888No Hit
TCGTCGCCGTCGCCGTGACCGCCGCCTGCGTCTGCACCTGCAACGTCACC11.3888888888888888No Hit
GCACAGGAAACCTTTTCCCCTTCGGACCTGCCCGGGGAACCGTTCTCTCT11.3888888888888888No Hit
AATTCCGATTAGGCAGCGCTACAAACGAACATGTGCTTAAACTAGACGGC11.3888888888888888No Hit
ACTCCACTTATGTACTACAGTGTCCCTTTGTCACGCGGTGGCAAGGAACA11.3888888888888888No Hit
AGGTGCCTTGCATTCCCACTCATCCAACCCGCATGGTTCCGCCGTAAATA11.3888888888888888No Hit
TATGTCGGGACAGATTCTCTCTGCCTTTTTCTCACTTACGCTCGTTTTCC11.3888888888888888No Hit
TAGGGCGCAACCAGTGAGCAGTTGACGGGCCAAAAGAAGACAACGCCAGA11.3888888888888888No Hit
TCCCTAGACCTTCCCCTACGCTACCACTCTATCGAGCAGTATTTCCAACA11.3888888888888888No Hit
CCACCGGGGCTGTCTCTTATACACATCTGACGCTGCCGACGAATAGAGAG11.3888888888888888No Hit
TGCATAATGAGGCACGGGCCCCTCCCCGCGCGGGGCTGGCGGGGTCCCGG11.3888888888888888No Hit
GGCCAATGATGAAGCCAAGGTGTTCAAGTGTGCAGCCTCGATAGCTCCAG11.3888888888888888No Hit
CATACTCTACAGAACATCCAACTCCTGCTTCTACAAGTCTTCTACCCGTC11.3888888888888888No Hit
TACAGTGGGACCACTGAGAAGCTCCTAACAACATCCACACACAGTTGAGG11.3888888888888888No Hit
TAGCTCTAGACGAGCCCCCCGATCCCCTCACCACCCTCCCCTACTGCCCC11.3888888888888888No Hit
AGGAGAAATACACTACAGGTCTCACGACACCAGCCCCGCAATCAGCTGGA11.3888888888888888No Hit
TCGGGTCCAAAAAAAACGGAAAAATTTCCACACAACGCCATACATGGTGG11.3888888888888888No Hit
ACTCCTCCACCACTATTAATTCTGCGTCCACTGCTCTTACAGCCAGCCTG11.3888888888888888No Hit
GAAAGCCTCAGACAATGAACCAGCGCCCGCCTCTAAGCACCCACCACCCC11.3888888888888888No Hit
TACTGGGGAGACGTTTGCTCTTCCGATCTTCCTGGGGTGACGCCGACTCT11.3888888888888888Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TAGAAAAAATAATTAACCGAAACCATCAAAGCAGTTGCGACTCCCACTCA11.3888888888888888No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers