FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s30.352000000d7e68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s30.352000000d7e68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11577426
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT1622461.4013995857110206No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA1354841.1702428501810334No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT1143520.9877152313476242No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA879780.759909845245394No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT223280.19285806706948505No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA158850.1372066640719621No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC449300.041.46776
GAGACGT422250.040.4019438
GGAGACG453350.040.186537
AGGGAGA208600.038.135425
AGACGTG414850.038.008669
CGATCTG332600.036.0694245
CAGGGAG125900.036.0251434
CCGATCT741900.034.2288934
TGGGAGA181500.033.9122355
TTCCGAT774300.033.1216962
TCCGATC791450.032.5419963
AGACGTT45300.028.7268439
TAGGGAG59300.027.7909154
CTGGGAG119500.027.7803254
CGATCTA156350.027.4902525
GATCTGG150350.027.2290136
CTTCCGA1024200.026.7883321
CGATCTT149350.024.1677635
GATCTGA107750.023.8896646
ACAGGGA61850.022.882563
Produced by FastQC (version 0.11.2)