FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s22.352000000d7d83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s22.352000000d7d83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8272152
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA96860.11709165885733241No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC412050.041.6100546
GGAGACG412850.040.4365437
AGGGAGA186800.039.9683955
GAGACGT398150.038.8876658
CAGGGAG114950.036.813724
CGATCTG280100.036.438825
AGACGTG391400.036.1608859
TGGGAGA152000.035.90195
CCGATCT676650.032.3857884
TTCCGAT693400.032.1103322
TCCGATC716300.031.1633343
GATCTGG126400.029.3457326
CTGGGAG106600.028.8707984
AGACGTT41800.027.8377049
CGATCTA134000.027.3977995
TAGGGAG54350.026.6527044
ACAGGGA47600.025.8424553
CCAGGGA69300.025.6318153
CTTCCGA971400.025.091671
CGATCTT136800.023.434665
Produced by FastQC (version 0.11.2)