FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s2.352000000d7b1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s2.352000000d7b1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11830011
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT537920.45470794574916285No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA439300.3713436952848142No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT389760.3294671492697682No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA291740.246610083456389No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT144080.12179194085280225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC379700.043.5283246
GAGACGT355000.042.2629788
AGACGTG344600.039.969019
GGAGACG404200.039.6684237
AGGGAGA190550.037.785365
TGGGAGA156200.034.966335
CAGGGAG123950.031.2280144
CCGATCT577300.028.6375454
TTCCGAT591850.028.3187032
TAGGGAG55350.027.715214
TCCGATC606450.027.6682663
CTTCCGA701500.024.819791
CGATCTG318600.024.6858375
AGACGTT49500.023.6031829
CGGGAGA112300.021.9916025
CTAGGGA33700.021.4213583
ACAGGGA59200.020.858593
ACGTGTG359500.020.2793410-11
GACGTGT355300.019.86405610-11
GATCTAG54500.019.7816566
Produced by FastQC (version 0.11.2)