FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s16.352000000d7cd4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s16.352000000d7cd4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGACAGATTTGTCATTGTGAGCATTTTCATCCCGAAGTTGCGGCTCAT16.25No Hit
TGCGACCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGG16.25No Hit
TAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGGTATTAAGG16.25No Hit
TCTGAACAGCTTCTTGGGAAGTAGCGACAGCTTGGTTTTTAGTGAGTTGT16.25No Hit
ATGGTTACAGTATGCCCATCGCAGTTCGCTACACGCAGGACGCTTTTTCA16.25No Hit
CGACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGCTTTGACTTATACC16.25No Hit
ACTCTTCAGGTTGGATACGCCAATCATTTTTATCGAAGAGCGCATAAATT16.25No Hit
TTAAAGCTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTAAATACGTT16.25No Hit
GAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTCTACTCTTTCTCA16.25No Hit
GATTCTGAACAGCTTCTTGGGAAGTAGCGACAGCTTGGTTTTTAGTGAGT16.25No Hit
GAGGGTGTCAATCCTGACGGTTATTTCCTAGACAAATTAGAGACAATACC16.25No Hit
GTACCCGACGACCAAAATTAGGGTCAACGCTACCTGTAGGAAGTGTCCGC16.25No Hit
GACTGCCTATGATGTTTATCCTTTGGATGGTCGCCATGATGGTGGTTATT16.25No Hit
CGACGCAATGGAGAAAGACGGAGAGCGCCAACGGCGTCGATCTCGAAGGA16.25No Hit
CATTGCGTCGTGGCCTTGCTATTGACTCTACTGTAGACGTTTTTAGTTTT16.25No Hit
TCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGTACTTATTCGC16.25No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)