FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s14.352000000d7c84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s14.352000000d7c84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7523421
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT145580.193502397380128No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT89580.1190681739065247No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG89150.1184966254048524No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA87510.11631676600312543No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA85050.11304697690053502No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT84490.11230263466579897No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC77920.10356990523327086No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC174300.041.1432236
GAGACGT161650.040.690488
GGAGACG181300.038.3758967
AGACGTG169000.035.52059
AGGGAGA88000.035.456965
CCGATCT324000.033.9185374
TTCCGAT339550.032.9526442
TCCGATC344100.032.5307163
TGGGAGA79750.028.6439655
CTTCCGA422100.028.2408071
TAGGGAG27100.027.3384594
CAGGGAG64300.026.9588054
CGATCTG209000.025.0411365
AGACGTT25050.021.0442779
TAGGACT80450.020.8385454
CTAGGAC81850.020.7722283
CGATCTA96600.020.2061445
CTAGGGA18350.020.1872563
TCTAGGA90000.019.7354872
CGGGAGA62100.019.7308985
Produced by FastQC (version 0.11.2)