FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s13.352000000d7c6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s13.352000000d7c6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12490683
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT348030.2786316809096828No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA278650.22308627958935473No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT259150.2074746433001302No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT247020.19776340493149971No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA180850.1447879191233978No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG145230.11627066350174767No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA144240.11547807273629472No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT137760.11029020590787549No Hit
TCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTT129900.10399751558821883No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT127150.10179587457307179No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT355750.036.7530484
TCCGATC363550.036.29113
TTCCGAT362950.036.285672
GGGAGAC152100.032.193016
CTTCCGA442150.031.4294471
GAGACGT141750.030.9923298
AGACGTG144400.027.9238999
GGAGACG179850.026.3281447
AGGGAGA91550.024.174625
CGATCTG280300.022.2471145
TAGGGAG30500.021.6444744
CTAGGAC138600.020.5941243
TAGGACT138800.020.4617984
TGGGAGA82850.020.3501635
TATGCTT87700.020.1453785
CGATCTA118300.020.113385
TCTAGGA153650.019.7824232
ATCTAGG147400.019.3324031
AGGACTA145150.019.2721585
GTATAGT93300.019.139841
Produced by FastQC (version 0.11.2)